国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (2): 220-222.

• 论著 • 上一篇    下一篇

经阴道注入重组白细胞介素17对正常妊娠CBA/J孕鼠的影响

柳红杰，曲洪美，马宏仲，殷伟红，于丽丽，周小娇，王文娟   

  1. 264000 山东省烟台市，青岛大学医学院附属烟台毓璜顶医院产科（柳红杰，曲洪美，殷伟红，于丽丽），麻醉科（马宏仲），生殖医学中心（周小娇，王文娟）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-15 发布日期:2015-04-15
  • 通讯作者: 王文娟

The Effect of Recombinant IL-17 Administered in the Vaginal Vaults on Normal Pregnancy CBA/J Mice

LIU Hong-jie，QU Hong-mei，MA Hong-zhong，YIN Wei-hong，YU Li-li，ZHOU Xiao-jiao，WANG Wen-juan   

  1. Department of Obstetrics（LIU Hong-jie，QU Hong-mei，YIN Wei-hong，YU Li-li），Department of Anesthesiology（MA Hong-zhong），Reproductive Medical Center（ZHOU Xiao-jiao，WANG Wen-juan），Yantai Yuhuangding Hospital，Qingdao University School of Medicine，Yantai 264000，Shandong Province，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-15 Online:2015-04-15
  • Contact: WANG Wen-juan

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摘要： 目的：观察经阴道注入重组白细胞介素17（rIL-17）对正常妊娠CBA/J孕鼠（CBA/J雌鼠×BALB/c雄鼠）妊娠结局的影响，探讨其在诱导母胎免疫排斥中的作用。方法：将6～8周龄清洁级CBA/J（雌）和BALB/c（雄）近交系小鼠分为3组。对照组：正常妊娠模型CBA/J孕鼠未作任何干扰。磷酸盐缓冲液（PBS）组：正常妊娠模型CBA/J孕鼠，于受孕第1天麻醉状态下经阴道注射50 μL PBS。rIL-17组：正常妊娠模型CBA/J孕鼠，于受孕第1天麻醉状态下经阴道注射50 μL rIL-17。受孕后第14天观察胚胎丢失率。通过实时荧光定量聚合酶链反应（qRT-PCR）方法、蛋白质印迹（Western blotting）方法检测鼠蜕膜转化生长因子β（TGF-β）和IL-10的表达。结果：给予正常妊娠模型孕鼠外源性rIL-17，能引起其胚胎丢失率明显增加，且蜕膜TGF-β和IL-10 mRNA及蛋白的表达均明显降低（P＜0.05）。而对照组与PBS组相比，胚胎丢失率及TGF-β和IL-10 mRNA及蛋白的表达差异均无统计学意义（均P＞0.05）。结论：经阴道注入rIL-17能够引起正常妊娠模型CBA/J孕鼠胚胎丢失率增加，并引起母胎界面免疫耐受细胞因子分泌异常。

关键词: 白细胞介素10, 白细胞介素17, 转化生长因子β, 妊娠, 动物

Abstract: Objective： To investigate the effect of transvaginal injected recombinant IL-17 on the pregnancy outcome in the CBA/J mice model （CBA/J×BALB/c） and its role in feto-maternal immune rejection. Methods：Six to eight-week-old female CBA/J and male BALB/c were divided into three different experimental groups. Mice of normal pregnancy（CBA/J×BALB/c） received no treatment were served as control group. 50 μL phosphate-buffered saline（PBS） or 50 μL recombinant IL-17 （rIL-17） was administered in the vaginal vaults of anesthetized pregnant CBA/J mice on Day 1 of pregnancy served as PBS group and rIL-17 group individually. The numbers of surviving and reabsorbed fetuses in each group were counted, and the expression of TGF-β and IL-10 in decidua was assessed by quantitative real-time PCR and western blotting on Day 14 of pregnancy. Ｒｅｓｕｌｔｓ：In this in vivo model of normal pregnancy, exogenous rIL-17 resulted in increased spontaneous resorption of fetuses and decreased expression of TGF-β and IL-１０ （Ｐ＜０．０５）． Ａｎｄ ｔｈｅｒｅ ｗａｓ ｎｏ ｓｔａｔｉｓｔｉｃａｌ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ＰＢＳ ｇｒｏｕｐ ａｎｄ ｃｏｎｔｒｏｌ ｇｒｏｕｐ （Ｐ＞０．０５）． Ｃｏｎｃｌｕｓｉｏｎｓ：Intravaginal rIL-17 can induce inflammation in feto-maternal interface resulting in abortion in normal pregnant CBA/J mice model.

Key words: Interleukin-10, Interleukin-17, Transforming growth factor beta, Pregnancy, animal