国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (3): 315-318.

• 论著 • 上一篇    下一篇

OGG1基因在五味子乙素预防苯并芘所致HTR8-SVneo细胞DNA氧化损伤中的作用

章艳燕，侯海燕，陈晓，陈亚琼   

  1. 300162 天津，中国人民武装警察部队后勤学院附属医院，天津市职业与环境危害防制重点实验室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-06-15 发布日期:2015-06-15
  • 通讯作者: 陈亚琼

Effect of OGG1 Gene on the Repair Schizandrin B Prevent Benzo[a]-pyrene Induced DNA Oxidative Stress in HTR8-SVneo Cells

ZHANG Yan-yan，HOU Hai-yan，CHEN Xiao，CHEN Ya-qiong   

  1. Affiliated Hospital, Logistics University of Chinese People′s Armed Forces，Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards，Tianjin 300162，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-06-15 Online:2015-06-15
  • Contact: CHEN Ya-qiong

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摘要： 目的：探讨8-羟基鸟嘌呤DNA糖基化酶1（OGG1）基因在五味子乙素（Sch B）预防苯并芘（BaP）致人绒毛膜滋养层细胞（HTR8-SVneo）DNA氧化损伤中的作用。方法：以HTR8-SVneo 细胞为载体，构建BaP氧化应激损伤模型，分为空白组、BaP组、Sch B不同剂量组（0.1，0.5，2.0 μmol/L），分别检测各组细胞内活性氧簇（ROS）和8-羟基脱氧鸟苷（8-OHdG）水平、人OGG1蛋白活性以及mRNA的水平。结果：与空白组比较，BaP组细胞内OGG1 mRNA和蛋白的表达水平降低，而细胞内ROS含量、8-OHdG的平均水平增加；然而与BaP组相比，Sch B组细胞内OGG1 mRNA和蛋白的表达水平升高，而细胞内ROS含量、8-OHdG的平均水平减少，差异均有统计学意义（均P＜0.05）。结论：Sch B能预防BaP致HTR8-SV neo细胞DNA的氧化损伤。这种抗氧化活性可能与OGG1基因表达水平改变，从而影响细胞内DNA的修复能力有关。

关键词: 苯并芘, 五味子, DNA糖基化酶类, 脱氧鸟苷

Abstract: Objective：To explore the effect of OGG1 gene on the repair Schizandrin B（Sch B） prevent Benzo[a]pyrene（BaP） induced DNA oxidative stress in HTR8-SVneo cells. Methods：BaP damage HTR8-SV neo cells for oxidative stress damage model，the cells were divided into blank group，20 μmol/L BaP group，and Sch B（0.1，0.5，2.0 μmol/L） groups to detect cellular ROS，cellular 8-OHdG，the levels of cellular OGG1 mRNA and OGG1 protein. Results：Compared with blank group， the levels of OGG1 mRNA and OGG1 protein in BaP group were reduced（P＜0.05），but the levels of ROS and 8-OHdG were increased（P＜0.05）. Compared with of BaP group，the levels of OGG1 mRNA and OGG1 protein were increased in Sch B groups（P＜0.05），but the levels of ROS and 8-OHdG were reduced（P＜0.05）. Conclusions：Sch B can protect HTR8-SVneo cells DNA from BaP oxidatie stress injuries. The antioxidant activity may change with OGG1 gene expression levels，which affects the repair of DNA in the cell.

Key words: Benzo（a）pyrene, Schisandra chinensis, DNA glycosylases, Deoxyguanosine