国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (3): 324-326.

• 论著 • 上一篇    下一篇

滋养细胞疾病中Cyclin E及其相关蛋白的表达

李娜，曲芃芃   

  1. 300100 天津市中心妇产科医院
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-06-15 发布日期:2014-06-15
  • 通讯作者: 曲芃芃

Expression of Cyclin E and Its Related Proteins in Gestational Trophoblastic Disease

LI Na，QU Peng-peng   

  1. Tianjin Central Hospital of Obstetrics and Gynecology，Tianjin 300100，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-06-15 Online:2014-06-15
  • Contact: QU Peng-peng

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摘要： 目的：探讨细胞周期蛋白E（Cyclin E）、细胞周期蛋白依赖激酶2（CDK-2）、P27KIP1和P21WAF1/CIP1在正常胎盘组织、葡萄胎和滋养细胞肿瘤（gestational trophoblastic neoplasia，GTN）组织中的表达，研究上述指标表达水平的相关性以及与妊娠滋养细胞疾病（gestational trophoblastic disease，GTD）的关系。方法：采用链霉素-过氧化物酶免疫组化方法检测30例正常胎盘组织、38例葡萄胎组织和9例GTN组织（侵蚀性葡萄胎8例，绒癌1例）中Cyclin E、CDK-2、P27KIP1和P21WAF1/CIP1的表达，分析其与GTD发生发展及预后的关系。结果：①Cyclin E、CDK-2在葡萄胎和GTN组织中的表达高于正常胎盘组织（P＜0.01）；P27KIP1和P21WAF1/CIP1在GTN组织中的表达低于葡萄胎和正常胎盘组织（P＜0.05），P27KIP1和P21WAF1/CIP1在发生恶性转变的葡萄胎组织中的表达低于未发生恶变者（P＜0.05）。②Cyclin E与P27KIP1、P21WAF1/CIP1的表达呈负相关（P＜0.05）；Cyclin E与CDK-2的表达呈正相关（P＜0.05）；P27KIP1与P21WAF1/CIP1的表达呈正相关（P＜0.05）。CDK-2表达随P27KIP1和P21WAF1/CIP1的表达降低呈增高趋势，但差异无统计学意义（P＞0.05）。结论：Cyclin E/CDK-2复合物及其抑制蛋白P27KIP1和P21WAF1/CIP1的异常表达参与了GTN的发生发展；P27KIP1和P21WAF1/CIP1可能成为预测葡萄胎恶变的有效标记物。

关键词: 妊娠滋养细胞肿瘤, 细胞周期蛋白E, 细胞周期蛋白依赖激酶2, P27KIP1, P21WAF1/CIP1

Abstract: Objective： To investigate the expression of Cyclin E，CDK-2 and their inhibitors P27KIP1 and P21WAF1/CIP1 in gestational trophoblastic disease（GTD）. Methods：Expression of the above indexes were determined immunohistochemically by specific antibodies for these proteins on formalin-fixed paraffin sections of 30 of normal placenta tissues，38 of hydatidiform moles and 9 of gestational trophoblastic neoplasms （GTN）， including 8 invasive moles and 1 choriocarcinoma. Results：①The expression of Cyclin E and CDK-2 in GTD was significantly higher than that in normal placentas（P＜0.01）. The expression of P27KIP1 and P21WAF1/CIP1 in GTN was significantly lower than that in hydatidiform moles and normal placenta tissues，respectively（P＜0.05）. Moreover，the expression of P27KIP1 and P21WAF1/CIP1 in hydatidiform moles which developed GTN was significantly lower than those spontaneously regressed.②Significant negative correlations between the Cyclin E and P27KIP1，P21WAF1/CIP1 staining indexes were observed（P＜0.05）. There was a positive correlation between the expression of CDK-2 and Cyclin E（P＜0.05）. Also，the expression of P27KIP1 and P21WAF1/CIP1 showed positive correlation （P＜0.05）. CDK-2 showed inverse expression pattern compared with P27KIP1 and P21WAF1/CIP1，but no statistical difference was observed（P＞0.05）. Conclusions：Disregulation of Cyclin E/CDK-2 complex and its inhibitors participated in the development of gestational trophoblastic tumor. P27KIP1 and P21WAF1/CIP1 may be used as effective indicators for the prognosis of hydatidiform moles.

Key words: Gestational trophoblastic neoplasms, Cyclin E, Cyclin-dependent kinase 2, P27KIP1, P21WAF1/CIP1