国际妇产科学杂志

国际妇产科学杂志 ›› 2010, Vol. 37 ›› Issue (2): 81-83.

• 综述 • 上一篇    下一篇

先天性疾病研究及产前诊断中Array CGH的应用

眭维国,欧明林,戴勇,陈洁晶   

  1. 541002 桂林，中国人民解放军第一八一医院中心实验室(眭维国，戴 勇，陈洁晶)；广西师范大学生命科学学院(欧明林)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-15 发布日期:2010-04-15
  • 通讯作者: 戴勇

The Application of Array CGH in Congenital Anomalies Research and Prenatal Diagnosis

SUI Wei-guo, OU Ming-lin, DAI Yong, CHEN Jie-jing   

  1. Key Laboratory, 181 Hospital of People’s Liberation Army, Guilin 541002, China （SUI Wei-guo, DAI Yong, CHEN Jie-jing）; College of Life Science, Guangxi Normal University, Guilin 541004, China （OU Ming-lin）
  • Received:1900-01-01 Revised:1900-01-01 Published:2010-04-15 Online:2010-04-15
  • Contact: DAI Yong

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摘要： 染色体微缺失、重复、扩增和非整倍体等基因组DNA失衡是导致胎儿发育迟缓、畸形、死胎、流产和其他先天性疾病的内在原因。微阵列－比较基因组杂交技术（Array CGH）利用基因芯片取代了传统比较基因组杂交技术的正常中期染色体作为杂交靶标，与分别采用不同荧光标记的待测DNA和参照DNA杂交，通过比较两种荧光强度的比率，检测出染色体基因拷贝数变化。Array CGH已成为一个重要的细胞遗传学研究工具，用于产前诊断和先天性疾病诊断染色体亚显微结构异常。

关键词: 微阵列－比较基因组杂交, 先天性疾病, 产前诊断

Abstract: Fetal hypoevolutism, abnormity, fetal death, miscarriage and other congenital anomalies are due to some internal reasons, e.g chromosome micro deletion, repeat, aneuploid and so on. Array comparative genomic hybridization （Array CGH） uses array to replace traditional metaphase chromosome as target crossing to samples and reference DNA labeled by different fluorescence, and the copy number alterations are detected by analysis of different fluorescence intensity. Array CGH is a powerful tool for cytogenetical study, and it has been used in prenatal diagnosis and congenital anomalies research for abnormal microarchitecture.

Key words: Array base comparative genomic hybridization, Congenital anomalies, Prenatal diagnosis