国际妇产科学杂志

国际妇产科学杂志 ›› 2016, Vol. 43 ›› Issue (2): 184-186.

• 论著 • 上一篇    下一篇

植酸酮对人宫颈癌细胞增殖的影响

高晓丽，王侃，田文艳，岳天孚   

  1. 300052 天津医科大学总医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-04-15 发布日期:2016-04-15
  • 通讯作者: 岳天孚

Effects of Phytic Acid Ketone on the Cell Proliferation of HeLa and Caski

GAO Xiao-li，WANG Kan，TIAN Wen-yan，YUE Tian-fu   

  1. Department of Obstetrics and Gynecology，Tianjin Medical University General Hospital，Tianjin 300052，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-04-15 Online:2016-04-15
  • Contact: YUE Tian-fu

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摘要： 目的：研究不同浓度植物提取物植酸酮在不同作用时间对两种宫颈癌细胞系增殖的影响。方法：以培养基稀释植酸酮妇科清洗装置Ⅱ型1号及2号至相应浓度，采用噻唑蓝（MTT）比色法检测不同药物浓度对宫颈癌HeLa细胞[腺癌，含人乳头状瘤病毒18（HPV18）基因]及Caski细胞（鳞癌，含HPV16基因）不同时间点的增殖抑制率。结果：植酸酮妇科清洗装置Ⅱ型1号及2号试剂对HeLa及Caski细胞增殖的抑制作用具有浓度依赖性和时间依赖性。不同浓度植酸酮妇科清洗装置Ⅱ型1号及2号试剂对HeLa及Caski细胞增殖抑制作用差异有统计学意义（P＜0.05），细胞增殖抑制率随给药浓度增加作用增强，浓度为5 860 mg/L、5 500 mg/L细胞增殖抑制率最高。同一给药浓度不同作用时间对细胞增殖抑制率差异具有统计学意义（P＜0.05）。结论：植酸酮对宫颈癌细胞系增殖具有抑制作用，可为临床上植酸酮抗HPV治疗宫颈病变及宫颈癌提供理论依据。

关键词: 植酸酮, 宫颈肿瘤, 癌, 人宫颈癌细胞, 乳头状瘤病毒科

Abstract: Objective：To investigate the proliferation inhibitory effects of different density Phytic Acid Ketone on cervical cancer cell HeLa and Caski. Methods：Use the culture medium to dilute the type II No.1 and No.2 Phytic Acid Ketone to different density. The HeLa (adeno carcinoma, HPV18 genome integrated) and Caski (squamous carcinoma, HPV16 genome integrated) cells were treated with different concentration and time of Mefordcare. The inhibitory effects were evaluated with MTT assay. Results：The growth of HeLa and Caski was inhibited by different concentration of Mefordcare Phytic Acid Ketone, which was concentration-time dependent. The inhibitory effect on HeLa and Caski of Phytic Acid Ketone No. 1 and No. 2 varied with the concentration used, and the inhibitory rate came to the peak when concentration was about 5 860 mg/L and 5 500 mg/L. The inhibitory rate of cell proliferation was significantly different with the treating time at the same concentration (P＜0.05). Conclusions：There were inhibitory effects from Phytic Acid Ketone on the growth of cervical cancer cell line HeLa and Caski. The result provides theoretical basis for the clinical application of Mefordcare Phytic Acid Ketone.

Key words: Phytic acid ketone, Cervical cancer, Cancer, Human cervical cancer cell, Human papillomavirus