国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (5): 533-535.

• 论著 • 上一篇    下一篇

早发型重度子痫前期分娩前后CD28/CTLA-4的相关研究

张娜娜，吕英璞，杨石芳，陈艳珺，张文真，李从伟   

  1. 050011 石家庄市第三医院产科（张娜娜，杨石芳，陈艳珺，李从伟）；石家庄市第一医院产科（吕英璞）；河北医科大学附属第二医院产科（张文真）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-10-15 发布日期:2015-10-15
  • 通讯作者: 张娜娜

The Study of the CD28/CTLA-4 Disturbance in Early-onset Severe Preeclampsia Prior to Delivery and after Parturition

ZHANG Na-na，LYU Ying-pu，YANG Shi-fang，CHEN Yan-jun，ZHANG Wen-zhen，LI Cong-wei   

  1. Department of Obstetrics，Third Hospital of Shijiazhuang，Shijiazhuang 050011，China（ZHANG Na-na，YANG Shi-fang，CHEN Yan-jun，LI Cong-wei）；Department of Obstetrics，First Hospital of Shijiazhuang，Shijiazhuang 050011，China（LYU Ying-pu）；Department of Obstetrics，Second Hospital，Hebei Medical University，Shijiazhuang 050000，China（ZHANG Wen-zhen）
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-10-15 Online:2015-10-15
  • Contact: ZHANG Na-na

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摘要： 目的：探讨白细胞分化抗原28/细胞毒性T淋巴细胞相关抗原4（CD28/CTLA-4）与早发型重度子痫前期发病的关系。方法：采用流式细胞技术分别测定30例早发型重度子痫前期患者分娩前后的CD28、CTLA-4、CD28/CTLA-4表达水平，同时测量各组受试者血压及肝肾功能。结果：早发型重度子痫前期患者分娩前CD3+CD4+CTLA-4+、CD3+CD8+CTLA-4+高于分娩后，CD3+CD4+CD28+/CD3+CD4+CTLA-4+、CD3+CD8+CD28+/CD3+CD8+CTLA-4+低于分娩后，差异有统计学意义（均P＜0.05）。CD3+CD4+CD28+/CD3+CD4+CTLA-4+、CD3+CD8+CD28/CD3+CD8+CTLA-4+两者无直线相关性（r=－0.037，P=0.844）。结论：早发型重度子痫前期患者分娩后较分娩前CTLA-4的表达下降，CD28/CTLA-4上升，可能提示CD28/CTLA-4免疫偏移与子痫前期的发生有关。

关键词: 先兆子痫, 抗原, CD28, T淋巴细胞亚群, Th1细胞, Th2细胞, 分娩, 细胞毒性T淋巴细胞相关抗原4

Abstract: Objective：To study the alteration of the leukocyte differentiation antigens 28/cytotoxic T lymphocyte antigen 4 (CD28/CTLA-4) in maternal blood lymphocyte from patients with early-onset severe preeclampsia to investigate the function of CD28/CTLA-4 in the pathogenesis of early-onset severe preeclampsia. Methods：Peripheral blood from 30 patients with early-onset preeclampsia (PE) [prior to delivery (PTD) and after parturition (AP)]. It applies flow cytometry to detect expression of CD28/CTLA-4 of peripheral lymphocytes in CD4+T and CD8+T cells. At the same time, to determine blood pressure and the functions of liver and kidney. Results：The CD3+CD4+CTLA-4+, CD3+CD8+CTLA-4+ cells in early-onset severe preeclampsia before delivery above after childbirth, CD3+CD4+CD28+/CD3+CD4+CTLA-4+, CD3+CD8+CD28+/CD3+CD8+ CTLA-4+ below after childbirth, was statistically significant (P＜0.05). There isn′t correlation between the CD3+CD4+CD28+/CD3+CD4+CTLA-4+ and CD3+CD8+CD28+/CD3+CD8+CTLA-4+ (r=-0.037, P=0.844). Conclusions：After parturition, the station about the decrease of CTLA-4 and the increase of CD28/CTLA-4 might be relation to early-onset preeclampsia.

Key words: Pre-eclampsia, Antigens, CD28, T-lymphocyte subsets, Th1 cells, Th2 cells, Cytotoxic T-lymphocyte-associated antigen 4