国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (5): 509-512.

• 论著 • 上一篇    下一篇

miR-200c过表达对OVCAR-3卵巢癌细胞生物学行为的影响

吴海湉，张越，林其德，张殊   

  1. 200127 上海交通大学医学院附属仁济医院妇产科 上海市妇科肿瘤重点实验室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 张殊

The Effect of miR-200c Over Expression on OVCAR-3 Epithelial Ovarian Cancer Biological Functions

WU Hai-tian，ZHANG Yue，LIN Qi-de，ZHANG Shu   

  1. Department of Obstetrics and Gynecology，Shanghai Key Laboratory of Gynecology Oncology，Renji Hospital，Shanghai Jiao Tong University, School of Medicine，Shanghai 200127，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-10-15 Online:2014-10-15
  • Contact: ZHANG Shu

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摘要： 目的：探讨miR-200c过表达对上皮性卵巢癌细胞OVCAR-3增殖、迁移和侵袭能力的影响。方法：构建逆转录病毒表达质粒pMSCVpuro-miR-200c，转染OVCAR-3卵巢癌细胞株，分别用细胞计数试剂盒（Cell Counting Kit-8，CCK8）和Transwell小室法检测过表达miR-200c的OVCAR-3细胞的增殖、迁移以及侵袭力改变，同时采用基因芯片检测转染前后OVCAR-3细胞的差异基因表达情况。结果：与未转染细胞相比，pMSCVpuro-miR-200c-OVCAR-3细胞的增殖、迁移及侵袭力下降（均P＜0.01）。通过基因芯片的检测，miR-200c过表达可引起OVCAR-3细胞中上调表达的基因有190个，下调表达的基因有166个。结论：miR-200c作为抑癌基因参与抑制上皮卵巢癌细胞的增殖、迁移和侵袭力。

关键词: 卵巢肿瘤, 细胞增殖, 细胞运动, 肿瘤侵润, miR-200c

Abstract: Objective： To investigate the effect of the miR-200c over-expression on the ability of proliferation, migration and invasion in OVCAR-3 ovarian cancer cells. Methods：Constructed retrovirus expression vector pMSCVpuro-miR-200c and transfected OVCAR-3 cells to overexpress miR-200c. By using CCK8 and Transwell methods to detect the ability of OVCAR-3 cells in proliferation and metastasis. Different expression genes involved in OVCAR-3 cells were detected by using gene chip before and after transfected with pMSCVpuro-miR-200c. Results：Compared with untransfected cells, the ability of proliferation, migration and invasion in pMSCVpuro-miR-200c transfected OVCAR-3 cell are significantly decreased（P＜0.01）. 190 up-regulated genes and 166 down-regulated genes were detected in OVCAR-3 cells after over-expressed miR-200c. Conclusions：As a tumor suppression gene，overexpression of miR-200c could inhibit the proliferation，migration and invasion of ovarian cancer cells.

Key words: Ovarian neoplasms, Cell proliferation, Cell movement, Neoplasm invasiveness, miR-200c