国际妇产科学杂志

国际妇产科学杂志 ›› 2009, Vol. 36 ›› Issue (5): 408-410.

• 论著 • 上一篇    下一篇

干预AE和NKCC离子通道对有核红细胞富集效果的观察

程宁;张丽娜;刘芳;徐向红;白亚娜   

  1. 730000 兰州，兰州大学生殖健康与出生缺陷研究中心(程宁,张丽娜,刘芳,徐向红);兰州大学公共卫生学院流行病与卫生统计学研究所(白亚娜);兰州大学基础医学院甘肃省新药临床前重点实验室(程宁)
  • 收稿日期:2009-03-12 修回日期:1900-01-01 出版日期:2009-10-15 发布日期:2009-10-15
  • 通讯作者: 程宁

The Study of the Nuclear Red Blood Cells′ Rich by Interfering Membrane Ionic Channels Selectively

CHENG Ning;ZHANG Li-na;LIU Fang;XU Xiang-hong;Ya-na   

  1. Center of Reproductive Health and Birth Defects, Lanzhou University, Lanzhou 730000, China（CHENG Ning, ZHANG Li-na, LIU Fang, XU Xiang-hong）; Institute of Epidemiology and Statistics, Medical School, Lanzhou University, Lanzhou 730000, China（BAI Ya-na）; Gansu Province Key Laboratory of Preclinical Study for New Medicine, Lanzhou University, Lanzhou 730000, China（CHENG Ning）
  • Received:2009-03-12 Revised:1900-01-01 Published:2009-10-15 Online:2009-10-15
  • Contact: CHENG Ning

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被引次数

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摘要： 目的：探讨联合干预阴离子通道（AE）和Na+/K+/2Cl-离子共转运体（NKCC）细胞膜离子通道对富集脐血中有核红细胞（NRBC
）的效果。方法：采用AE钙离子抑制剂维拉帕米（verapamil）和NKCC通道活性特异性抑制剂呋噻米（ferosemide）联合干预细胞膜，用流式细
胞技术观察脐血中胎儿NRBC的富集效果。结果：流式细胞仪检测NRBC的平均检出率干预前为2.4%，干预后为9.7%（u=3.19，P <0.05），联合干
预能提高NRBC的富集率4倍。结论：维拉帕米和呋噻米的干预能有效提升NRBC的富集率。

关键词: 胎儿有核红细胞, 离子通道, 维拉帕米, 呋噻米, 干预, 流式细胞仪

Abstract: Objective: To investigate the effectiveness of the Nuclear Red Blood Cells ′ rich by effecting
membrane ionic channels-Anion Exchanger and Na+/K+/2Cl- Co-transporter in umbilical cord blood. Methods: Make the optimal
interfering condition from RBCs on the umbilical cord blood，the rate of cells′ enrichment by discontinuous density
centrifugation on different densities were determined by Flow Cytometry（FCM）. Results: The average purity of NRBCs
enrichment in the umbilical cord blood could take from 2.4% to 9.7% （u=3.19, P<0.05） by interfering, which means to increase
about 4 times of enrichment of NRBC. Conclusions: Both Verapamil and Furosemide could promote the rate of NRBC′enrichment
about 4 times.

Key words: Nuclear red blood cells, Ionic channels, Verapamil, Furosemide, Interfering, Flow cytometry