国际妇产科学杂志

国际妇产科学杂志 ›› 2016, Vol. 43 ›› Issue (5): 539-541.

• 论著 • 上一篇    下一篇

表没食子酸酯诱导卵巢癌SKOV3细胞凋亡及机制的研究

周菲菲，董天睿，王晶，刘艳秋，倪锦红   

  1. 150001 哈尔滨医科大学附属第一医院妇科（周菲菲，董天睿，王晶，倪锦红）；红兴隆中心医院（刘艳秋）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-10-15 发布日期:2016-10-15
  • 通讯作者: 倪锦红

EGCG Induce the Apoptosis of Ovarian Cancer SKOV3 Cells and Its Mechanism

ZHOU Fei-fei，DONG Tian-rui，WANG Jing，LIU Yan-qiu，NI Jin-hong   

  1. Department of Gynecology，The First Affiliated Hospital of Harbin Medical University，Harbin 150001，China（ZHOU Fei-fei, DONG Tian-rui，WANG Jing，NI Jin-hong）；Hong Xinglong Center Hospital，Harbin 150001，China（LIU Yan-qiu）
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-10-15 Online:2016-10-15
  • Contact: NI Jin-hong

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摘要： 目的：探讨表没食子酸酯（EGCG）对人卵巢癌SKOV3细胞凋亡的影响及其作用机制。方法：SKOV3细胞分为4组，即阴性对照组（以正常培养液培养）、EGCG低浓度组（1 μmol/L EGCG）、EGCG中浓度组（10 μmol/L EGCG）和EGCG高浓度组（100 μmol/L EGCG）。应用四甲基偶氮唑盐（MTT）比色法测定各组SKOV3细胞的增殖抑制率，Hoechs染色观察细胞核凋亡，电镜观查细胞形态，蛋白质印迹（Western blot）检测Caspase-3表达。结果：细胞增殖抑制实验结果显示，EGCG低、中、高浓度组SKOV3细胞的增殖率为（81.33±3.71）%、（67.00±7.21）%和（54.67±3.84）%，与阴性对照组（101.00±3.06）%比较差异具有统计学意义（P＜0.05）。Hoechst33258染色结果显示，EGCG低、中、高浓度组凋亡细胞比例分别为（16.77±2.48）%、（24.33±3.70）%和（39.67±4.11）%，与阴性对照组[（2.00±1.45）%]相比差异均有统计学意义（Ｐ＜０．０５）。电镜观察结果显示，随着EGCG作用浓度的增加，SKOV3细胞超微结构损害程度逐渐明显。此外，EGCG可以升高Caspase-3表达水平。结论：EGCG通过增强Caspase-3表达诱导卵巢癌SKOV3细胞凋亡。

关键词: 表没食子酸酯, 没食子酸, 儿茶素, 卵巢肿瘤, 细胞凋亡, 半胱氨酸天冬氨酸蛋白酶3

Abstract: Objective：To investigate the effect and mechanism of EGCG on human ovarian cancer SKOV3 cells′ apoptosis. Methods：SKOV3 cells were divided into four groups. Negative control group: cultured with normal culture medium; EGCG low dose group: cultured with 1 μmol/L EGCG; EGCG middle dose group: cultured with 10 μmol/L EGCG; EGCG high dose group: cultured with 100 μmol/L EGCG. Cell proliferation inhibition rate of each group of SKOV3 cells were test by MTT assay, nuclei apoptosis were checked by Hoechs staine, cells morphology were investigated by electron microscope, Caspase-3 expression was detected by Western-blot. Results：Cell proliferation inhibition test results showed that the inhibition rate of EGCG low, medium and high concentration group on SKOV3 cells was (81.33±3.71)%, (67.00±7.21)% and (54.67±3.84)%, the difference was statistically significant (P＜0.05) compared with the negative control group (101.00±3.06)%. Hoechst33258 staining results showed that, apoptosis cell percentage of EGCG low, medium and high concentration group was (16.77±2.48)%, (24.33±3.70)% and (39.67±4.11)%, the difference was statistically significant (P＜0.05) compared with the negative control group [(2.00±1.45)%]. Electron microscopy showed that with the concentration of EGCG elevated, the damage on ultrastructure of SKOV3 cell became clear. In addition, EGCG could raised Caspase-3 expression levels. Conclusions：EGCG induced ovarian cancer SKOV3 cells apoptosis by enhancing the expression of Caspase-3 gene.

Key words: EGCG, Gallic acid, Catechin, Ovarian neoplasms, Apoptosis, Caspase 3