国际妇产科学杂志 ›› 2022, Vol. 49 ›› Issue (6): 713-716.doi: 10.12280/gjfckx.20220627

• 普通妇科疾病及相关研究:论著 • 上一篇    下一篇

DNA探针法诊断阴道炎价值的探讨

齐文慧1, 王辰1(), 薛凤霞1   

  1. 300052 天津医科大学总医院妇产科,天津市女性生殖健康与优生重点实验室
  • 收稿日期:2022-08-02 出版日期:2022-12-15 发布日期:2023-01-11
  • 通讯作者: 王辰 E-mail:wangchener32@163.com
  • 基金资助:
    国家自然科学基金(82101705);天津市卫生健康科技青年项目(QN20034);天津市卫生健康科技人才培育项目(KJ20003);天津市医学重点学科(专科)建设项目资助(TJSYXZDXK022)

Clinical Value of DNA Probe in Diagnosis of Vaginitis

QI Wen-hui1, WANG Chen1(), XUE Feng-xia1   

  1. Department of Obstetrics and Gynecology, Tianjin Key Laboratory of Female Reproductive Health and Eugenics, Tianjin Medical University General Hospital, Tianjin 300052, China
  • Received:2022-08-02 Published:2022-12-15 Online:2023-01-11
  • Contact: WANG Chen E-mail:wangchener32@163.com

摘要:

目的: 评估DNA探针法诊断阴道炎的临床价值。方法:纳入714例天津医科大学总医院妇产科门诊以阴道炎症状就诊的育龄期女性,同时行阴道微生态检测和DNA探针法诊断。以阴道微生态检测为对比标准,探讨DNA探针法诊断细菌性阴道病、外阴阴道假丝酵母菌病和阴道毛滴虫病的价值。结果:DNA探针法诊断细菌性阴道病的敏感度为98.32%,特异度为87.39%,约登指数为0.86,阳性预测值为60.93%,阴性预测值为99.61%,一致性检验Kappa值为0.69(P<0.001)。DNA探针法诊断外阴阴道假丝酵母菌病的敏感度为92.80%,特异度为97.39%,约登指数为0.90,阳性预测值为89.58%,阴性预测值为98.24%,一致性检验Kappa值为0.89(P<0.001)。DNA探针法诊断阴道毛滴虫病的敏感度为100%,特异度为99.86%,约登指数为0.99,阳性预测值为87.5%,阴性预测值为100%,一致性检验Kappa值为0.93(P<0.001)。结论:DNA探针法技术操作简便,可一次性检测3种阴道炎致病微生物,与阴道微生态检测法具有较好的一致性。同时克服了传统阴道微生态检测方法的局限性,具有很高的临床应用价值。

关键词: 阴道炎, 阴道病,细菌性, 念珠菌病,外阴阴道, 滴虫性阴道炎, DNA探针, 阴道微生态检测, 诊断性试验

Abstract:

Objective: To evaluate the clinical value of DNA probe in diagnosis of vaginitis. Methods: A total of 714 women of childbearing age with vaginitis symptoms in the Department of Obstetrics and Gynecology of Tianjin Medical University General Hospital were enrolled and diagnosed by vaginal microecological detection and DNA probe technology. To explore the clinical value of DNA probe in the diagnosis of bacterial vaginosis, vulvovaginal candidiasis and vaginal trichomoniasis by comparing vaginal microecological detection. Results: The sensitivity, specificity, Youden index, positive predictive value and negative predictive value of DNA probe in the diagnosis of bacterial vaginosis were 98.32%, 87.39%, 0.86, 60.93% and 99.61%, respectively. The Kappa value of consistency test was 0.69 (P<0.001). The sensitivity, specificity, Youden index, positive predictive value and negative predictive value of DNA probe in the diagnosis of vulvovaginal candidiasis were 92.80%, 97.39%, 0.90, 89.58% and 98.24%, respectively. The Kappa value of consistency test was 0.89 (P<0.001). The sensitivity, specificity, Youden index, positive predictive value and negative predictive value of DNA probe in the diagnosis of vaginal trichomoniasis were 100%, 99.86%, 0.99, 87.5% and 100%, respectively. The kappa value of consistency test was 0.93 (P<0.001). Conclusions: DNA probe technique is easy to operate and can detect three kinds of pathogenic microorganisms of vaginitis at one time, and has good consistency with vaginal microecological detection method. At the same time, it overcomes the limitation of traditional vaginal microecological detection method and has high clinical application value.

Key words: Vaginitis, Vaginosis, bacterial, Candidiasis, vulvovaginal, Trichomonas vaginitis, DNA probes, Vaginal microecological detection, Diagnostic test