国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (1): 63-65.

• 论著 • 上一篇    下一篇

Twist2和E-cadherin在宫颈鳞癌中的表达及意义

葛艳，杨永秀，胡乐，刘畅，朱骏   

  1. 730000 兰州大学第一临床医学院（葛艳，胡乐）；兰州大学第一医院妇产科（杨永秀，刘畅），病理科（朱骏）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-02-15 发布日期:2014-02-15
  • 通讯作者: 杨永秀

Expression and Significance of Twist2 and E-cadherin in Cervical Squamous Cell Carcinoma

GE Yan，YANG Yong-xiu，HU Le，LIU Chang，ZHU Jun   

  1. The First Clinical Medicine College of Lanzhou University，Lanzhou 730000，China（GE Yan，HU Le）；Department of Obstetrics and Gynecology（YANG Yong-xiu，LIU Chang），Department of Pathology（ZHU Jun），the First Hospital of Lanzhou University，Lanzhou 730000，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-02-15 Online:2014-02-15
  • Contact: YANG Yong-xiu

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摘要： 目的：研究Twist2与E-cadherin在宫颈上皮内瘤样变（CIN）及宫颈鳞状细胞癌中的表达情况，探讨其在宫颈癌变过程中的作用及意义。方法：应用蛋白质免疫印迹（Western blot）和免疫组织化学法检测Twist2、E-cadherin在正常宫颈组织（对照组）、CINⅠ组、CINⅡ～Ⅲ组、宫颈鳞癌组中的表达情况。结果：Twist2和E-cadherin在4组中阳性表达率比较差异有统计学意义（χ2=48.163，P=0.000；χ2=43.169，P=0.000）。宫颈鳞癌Twist2、E-cadherin的阳性表达率在不同分化程度、临床分期、有无淋巴结转移组间差异有统计学意义（P＜0.05），在不同年龄间差异无统计学意义（P＞0.05）。Twist2与E-cadherin在宫颈鳞癌组织中的表达呈负相关（r=-0.401，P=0.003）。对照组、CIN组、宫颈鳞癌组间Twist2、E-cadherin的相对表达量差异有统计学意义（F=652.225，P=0.000；F=299.112，P=0.000）。结论：Twist2、E-cadherin可能共同参与了宫颈鳞状细胞癌的恶性转化过程，联合检测Twist2与E-cadherin有助于判断宫颈癌的恶性程度及其预后。

关键词: Twist转录因子, 钙黏着糖蛋白类, 宫颈肿瘤, 宫颈上皮内瘤样病变, 印迹法, 蛋白质, 免疫组织化学

Abstract: Objective：Study Twist2 and E-cadherin expression in cervical intraepithelial neoplasia and cervical squamous cell carcinoma，and explore their roles and significance in the process of cervical cancer. Methods：Using western blot （Western blot） and immunohistochemistry method to detecte Twist2，E-cadherin expression in normal cervical tissue （control group），group CINⅠ，CINⅡ-Ⅲ group，cervical squamous cell carcinoma. Results：The differences of the positive expression rate of Twist2 and E-cadherin in four groups were statistically significant （χ2=48.163，P=0.000；χ2=43.169，P=0.000）. The positive expression rate of Twist2，E-cadherin in cervical squamous cell differentiation，clinical stage，lymph node metastasis between the groups，the difference was statistically significant （P＜0.05），no statistically significant difference between the different age groups（P＞0.05）. Twist2 and E-cadherin expression was negatively correlated （r=-0.401，P=0.003） in cervical squamous cell carcinoma. Control group，CIN group，cervical squamous cell carcinoma relative expression levels of Twist2，E-cadherin difference was statistically significant（F=652.225，P=0.000；F=299.112，P=0.000）. Conclusions：Twist2，E-cadherin may participate in the process of malignant transformation of cervical squamous cell carcinoma，joint detection Twist2，E-cadherin help to determine the degree of malignancy of cerical cancer and its prognosis.

Key words: Twist transcription factor, Cadherins, Uterine cervical neoplasms, Cervical intraepithelial neoplasia, Blotting, western