国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (2): 191-193.

• 论著 • 上一篇    下一篇

组蛋白去乙酰化酶抑制剂MS-275对人宫颈癌SiHa细胞

闫洪亮，吴维光，王筝，孙兆翎，唐雅娟   

  1. 063000 河北省唐山市，河北联合大学（闫洪亮）；中国人民武装警察部队后勤学院附属医院妇产科（闫洪亮，吴维光，王筝，孙兆翎，唐雅娟）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-04-15 发布日期:2014-04-15
  • 通讯作者: 吴维光

MS-275 on Migration and Invasion in Human Cervical Carcinoma Cell Line SiHa

YAN Hong-liang，WU Wei-guang，WANG Zheng，SUN Zhao-ling，TANG Ya-juan   

  1. Hebei United University，Tangshan 063000，Hebei Province，China（YAN Hong-liang）；Department of Obstetrics and Gynecology，the Affiliated Hospital of the Chinese People′s Armed Forces Logistic College，Tianjin 300162，China（YAN Hong-liang，WU Wei-guang，WANG Zheng，SUN Zhao-ling，TANG Ya-juan）
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-04-15 Online:2014-04-15
  • Contact: WU Wei-guang

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摘要： 目的：观察组蛋白去乙酰化酶抑制剂MS-275对人宫颈癌SiHa细胞迁移和侵袭的作用，并探讨其作用的可能机制。方法：应用不同浓度MS-275体外作用于人宫颈癌SiHa细胞，通过划痕实验检测细胞迁移能力，Transwell侵袭实验检测细胞侵袭能力。蛋白质印迹（Western blot）法检测细胞内组蛋白H4乙酰化水平，实时定量聚合酶链反应（PCR）方法检测细胞核因子κB（NF-κB）和尿激酶型纤溶酶原激活剂（uPA）基因mRNA表达。结果：经MS-275作用后，随MS-275浓度的增加，人宫颈癌SiHa细胞迁移速度降低，穿过Transwell滤膜的细胞数量减少。同时细胞内组蛋白H4乙酰化水平增加，细胞NF-κB和uPA基因mRNA表达降低。结论：MS-275可抑制人宫颈癌SiHa细胞迁移和侵袭能力，提高组蛋白乙酰化水平，降低NF-κB和uPA基因表达可能是其作用机制之一。

关键词: 宫颈肿瘤, 组蛋白脱乙酰基酶类, 肿瘤, 鳞状细胞, 肿瘤转移

Abstract: Objective：To observe the histone deacetylase inhibitor MS-275 on migration and invasion activity in human cervical carcinoma cell line SiHa，and to investigate the possible mechanism. Methods：SiHa cells were treated with different concentration of histone deacetylase inhibitor MS-275. The cell migration was estimated by wound-healing assay and the cell invasion was estimated by Transwell assay. Furthermore，Western blot was used to detect the acetyl level of histone H4 and real time PCR was used to detect mRNA of NF-κB and uPA gene. Results：After treatment with MS-275，With the increasing of concentration，the SiHa cell migration and invasion activity decreased. Meanwhile，the acetyl level of histone H4 increased，and the mRNA of NF-κB and uPA decreased in SiHa cells. Conclusions：The MS-275 could effectively inhibit the cell migration and invasion activity of SiHa cells，and the acetyl level of histone increasing to inhibite NF-κB and uPA gene expression may be one of its mechanisms.

Key words: Uterine cervical neoplasms, Histone deacetylases, Neoplasms, squamous cell, Neoplasm metastasis