Abstract: 【Abstract】  Objective：To investigate the expression profiles of Toll-like receptors (TLRs) in eutopic endometrium (EU) and ectopic endometrium (EC) and its implication in the inflammatory pathogenesis of adenomyosis. Methods：A total of 24 adenomyosis patients who underwent laparoscopy were recruited in this study. The mRNA and protein expressions of TLRs, and the mRNA expression of IL-6 and IL-8 in EU and EC of adenomyosis patients, and the control endometrium without adenomyosis (CE) from healthy women were detected. Results：The mRNA expression of IL-6, IL-8 in EU was significantly higher than that in CE, and was the highest in EC (P＜0.01). The mRNA expression of TLR1-6, 8, 9 in EU was significantly higher than that in CE (P＜0.05), and were the highest in EC except TLR2 (P＜0.01). The mRNA expression of TLR7 in EU was significantly higher than that in CE (P＜0.05), but there was no significant difference in TLR7 expression between EC vs. CE, EU vs. EC. The protein expression of TLR1, 4-6, 8, 9 in EU was significantly higher than that in CE, and was the highest in EC (P＜0.01). The protein expression of TLR2 in EC was significantly higher than that in CE (P＜0.05), but there was no significant difference between EU vs. CE, EU vs. EC. The protein expression of TLR3, 7 in EC was significantly higher than that in EU and CE (P＜0.05), but there was no significant difference between EU vs. CE. Pearson correlation analysis showed that the expression of TLR2, 4, 5 and 9 in EU and EC was positively correlated with that of IL-6 and IL-8 (P＜0.05). Conclusions：Our results showed that adenomyosis was a state of inflammatory pathology. High expression of TLRs in EU and EC were positively correlated with IL-6 and IL-8, which may be involved in the inflammatory pathogenesis of adenomyosis.

Key words: 【Keywords】 , Toll-like receptors, Interleukin-6, Interleukin-8, Adenomyosis