国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (2): 226-228.

• 论著 • 上一篇    下一篇

子宫内膜蜕膜化过程中miR-203的表达及其意义

王煜，陈秀娟，李光鹏，王丽岩   

  1. 010050 呼和浩特，内蒙古医科大学实验动物中心（王煜，李光鹏）；内蒙古医科大学附属医院生殖中心（王煜，陈秀娟，王丽岩）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-15 发布日期:2015-04-15
  • 通讯作者: 王丽岩

Expression and Significance of miRNA-203 in Endometrial Decidualization

WANG Yu，CHEN Xiu-juan，LI Guang -peng，WANG Li-yan   

  1. Research Center for Laboratory Animal Science of Inner Mongolia University，Hohhot 010050，China (WANG Yu，LI Guang -peng)；Reproductive Center，Inner Mongolia Medical University Affiliated Hospital，Hohhot 010050，China (WANG Yu ，CHEN Xiu-juan ，WANG Li-yan)
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-15 Online:2015-04-15
  • Contact: WANG Li-yan

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摘要： 目的：了解子宫内膜和蜕膜组织中miR-203的表达，探讨其在子宫内膜蜕膜化中的意义。方法：选取分泌期子宫内膜组织14例（分泌期组），正常妊娠早期蜕膜组织16例（早孕组），自然流产蜕膜组织18例（流产组），利用定量聚合酶链反应（qPCR）技术检测3组miR-203的表达量以及候选靶基因胰岛素样生长因子2受体（IGF2R）的mRNA表达量。结果：分泌期组miR-203的表达量高于早孕组和流产组，差异有统计学意义（P＜0.05）。IGF2R在早孕组表达量最高，在流产组表达量最低，差异具有统计学意义（P＜0.05）。结论：miR-203的表达变化可能参与子宫内膜蜕膜化，IGF2R的差异表达对蜕膜化以及早期妊娠的评估可能有一定的指导意义。

关键词: 子宫内膜, 蜕膜, 微RNAs, 受体, IGF2型

Abstract: Objective：To detect the expression of miR-203 in endometrium and decidua， and explore its significance during decidualization of endometrium. Methods：14 secretory endometria， 16 early pregnancy deciduas and 18 spontaneous miscarriage deciduas were collected in the study. MiR-203 expression level was detected by quantitative polymerase chain reaction（qPCR）. And IGF2R was predicted as the target gene of miR-203 respectively. Furthermore，the miRNA expression of IGF2R was detected by fluorescence quantitative PCR. Results：The expression of miR-203 in secretory endometrium was higher than that in spontaneous miscarriage decidua and early pregnancy decidua （P＜0.05）. Moreover，IGF2R was increased in early pregnancy，comparing with secretory endometrium and spontaneous miscarriage decidua（P＜0.05）. Conclusions：The expression of miR-203 may be associated with decidualization by targeting IGF2R. And IGF2R might associate with early pregnancy maintenance.

Key words: Endometrium, Decidua, MicroRNAs, Receptor, IGF type 2