Abstract: Anti-Müllerian hormone（ＡＭＨ） can regulate the growth and differentiation of germ cells. AMH is an ideal marker to evaluate the ovarian reserve, which is of great clinical significance for predicting the female menopause age and indicating fertility preservation. It retains its high predictive value for controlled ovarian hyperstimulation, and helps to identify women who are easy to be ovarian stimulation abnormalities in assisted reproductive technology（ＡＲＴ）；It also can act as a reliable follow-up marker of the ovarian granular cell tumor and used as an adjuvant therapy for epithelial ovarian cancer, cervical cancer and endometrial carcinoma；and may be correlated with infertility, insulin resistance and other clinical manifestations of polycystic ovarian syndroｍｅ（ＰＣＯＳ） and be useful to establish treatment protocols and to define the best strategy for ovulation induction in infertile women；and it also helps to distinguish different sexual differentiation dysplasia. This paper briefly introduces the regulation of follicle growth initiation and some related influence factors of AMH, expounds the gynecological clinical application, mainly in the following five aspects: the ovarian function, the ovarian reactivity, the gynecological oncology, PCOS and the sexual differentiation dysplasia.