关键词: 卵巢癌, 泰素帝, 顺铂, 赫赛汀, 抑制作用

Abstract: Objective：1.To study the anti-tumor effect of taxol drugs Taxotere on nude mice xenografts of human ovarian cancer SKOV3 cells and explore its possible mechanism; 2.To compare the anti-tumor effect of Taxotere with the combination of DDP,Herceptin on nude mice xenografts of human ovarian cancer SKOV3 cells.Methods：An animal model with human ovarian cancer SKOV3 cells involved in nude mice was established and the mice were randomized into 8 groups: ①DDP group,②Herceptin group,③Taxotere group,④DDP plus Herceptin group,⑤DDP plus Taxotere group,⑥Herceptin plus Taxotere group,⑦DDP plus Herceptin plus Taxotere group,⑧NS group，There are 4 mice in every group. The mice were administrated respectively with Cisplatin(3mg/kg)、Herceptin (20mg/kg)、Taxotere（5mg/kg） via caudal vein injection once a week for consecutive six weeks. The size of the tumor and mice weight were measured weekly, then the mice were killed a week after last treatment. The inhibition ratio of tumor growth was calculated and tumor tissues were observed by using HE staining. The apoptotic index was analyzed by using Tunel technique. The Ki-67 in xenograft tumors were analyzed by using immunohistochemical staining.Results：Taxotere can significantly inhibit the growth of xenografts of human ovarian cancer SKOV3 cells in nude mice, and the inhibition effect is more prominent when it was joint with Cisplatin or Herceptin respectively, while the inhibition effect is the most obvious when the three drugs above is joint together; 2. The tumor cell apoptotic index of Taxotere group was significantly increased than the control group, and the apoptotic index is higher when Taxotere was joint with Cisplatin or Herceptin respectively, while the apoptotic index is the highest when the three drugs above is joint together;3. The Ki-67 ratio in xenograft tumors of Taxotere group was significantly reduced than the control group, and Ki-67 ratio in xenograft tumors is lower when Taxotere was joint with Cisplatin or Herceptin respectively, while the Ki-67 ratio in xenograft tumors is the lowest when the three drugs above is joint together.Conclusion 1.Taxotere can obviously inhibit the growth of xenografts of human ovarian cancer SKOV3 cells in nude mice; Down-regulation of Ki-67 in SKOV3 cells,and induction of tumor cell apoptosis might be one of its possible mechanisms;2.There are synergistic mechanism of combinative action of Taxotere and DDP,Herceptin respetively,while the three drugs’ combination can more effectively inhibit the growth of tumor.

Key words: Ovarian cancer, Taxotere, DDP, Herceptin, Inhibition