国际妇产科学杂志

国际妇产科学杂志 ›› 2016, Vol. 43 ›› Issue (5): 585-589.

• 论著 • 上一篇    下一篇

五味子乙素降低苯并芘对HTR-8/SVneo细胞毒性作用的体外研究

董渠龙，侯海燕，陈俊，高秀霞，陈亚琼   

  1. 300162 天津，中国人民武装警察部队后勤学院附属医院妇产科（董渠龙，侯海燕，陈俊，高秀霞，陈亚琼）；中国医学科学院 北京协和医学院（侯海燕）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-10-15 发布日期:2016-10-15
  • 通讯作者: 陈亚琼

Schisandrin B Attenuating Benzo(a)pyrene-induced HTR-8/SVneo Cells Damage In Vitro

DONG Qu-long，HOU Hai-yan，CHEN Jun，GAO Xiu-xia，CHEN Ya-qiong   

  1. Department of Obstetrics and Gynecology，Affiliated Hospital of Medical College of Chinese People′s Armed Police Forces，Tianjin 300162，China（DONG Qu-long, HOU Hai-yan, CHEN Jun, GAO Xiu-xia, CHEN Ya-qiong）；Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100730，China（HOU Hai-yan）
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-10-15 Online:2016-10-15
  • Contact: CHEN Ya-qiong

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摘要： 目的：探索苯并芘[Benzo（a）pyrene，BaP]是否对人早孕胎盘绒毛膜外滋养层细胞HTR-8/SVneo具有毒性作用以及五味子乙素（Schisandrin B，Sch B）是否可以降低BaP的毒性作用。方法：以HTR-8/SVneo细胞为载体，构建BaP对HTR-8/SVneo细胞的染毒模型和Sch B对HTR-8/SVneo细胞的保护模型，利用MTS细胞增殖实验检测不同浓度的BaP单独给药和Sch B联合BaP给药对HTR-8/SVneo细胞增殖的影响。结果：0.01，0.1 μmol/L浓度的BaP并不抑制HTR-8/SVneo细胞的增殖（P＞0.05），而1，10，100 μmol/L浓度的BaP对HTR-8/SVneo细胞的增殖有抑制作用（P＜0.05）且随着其作用时间（6，12，24，36和48 h）的延长而增大，但作用24 h后其抑制作用趋于平衡，通过绘制HTR-8/SVneo细胞的生长曲线发现，20 μmol/L的BaP对HTR-8/SVneo细胞作用24 h后的抑制作用最佳，其抑制率达到24.95%；0.01，0.1，1 μmol/L浓度的Sch B并未抑制HTR-8/SVneo细胞的增殖（P＞0.05），高于10 μmol/L的Sch B抑制HTR-8/SVneo细胞的增殖（P＜0.05）。0.25，0.5，1，2 μmol/L的Sch B可以降低BaP的毒性作用且对HTR-8/SVneo细胞没有毒性作用，且其保护作用随着浓度增加而增大（P＜0.05），其中2 μmol/L的Sch B对HTR-8/SVneo细胞的保护作用最强，保护率达到17.84%。结论：BaP对HTR-8/SVneo细胞具有毒性作用，而一定浓度范围内的Sch B可以有效地降低BaP的这种毒性作用。

关键词: 苯并芘, 五味子素, 五味子乙素, 滋养层, 滋养层细胞, 比色法

Abstract: Objective: To investigate whether administering of benzo(a)pyrene (BaP) and schisandrin B (Sch B) to human trophoblast HTR-8/SVneo cells has cytotoxicity and cytoprotection, respectively. Methods: By HTR-8/SVneo cells culture in vitro, MTS cell proliferation assay was performed to detect the effect of different concentration of BaP-alone, Sch B-alone and Sch B combining with BaP treatment on cell proliferation. Results: 0.01 and 0.1 μmol/L BaP did not inhibit HTR-8/SVneo cell proliferation (P＞0.05), but 1, 10 and 100 μmol/L BaP all inhibited HTR-8/SVneo cell proliferation (P＜0.05). The inhibition rate were presented to be escalated accompanied by escalating BaP time exposure (6, 12, 24, 36 and 48 h), and 24 h BaP exposure were presented to be a saturation, especially for 20 μmol/L BaP with 24 h exposure which presented a suitable inhibition rate (24.95%). Besides, 0.01, 0.1 and 1 μmol/L Sch B did not inhibit HTR-8/SVneo cell proliferation (P＞0.05), but the level of SchB higher than 10 μmol/L inhibited HTR-8/SVneo cell proliferation (P＜0.05). 0.25, 0.5, 1 and 2 μmol/L Sch B all attenuated the cytotoxicity induced by BaP in HTR-8/SVneo cells with a dose-effect relationship, especially for 2 μmol/L Sch B which presented a highest protection rate (17.84%). Conclusions: BaP can directly induce cytotoxicity in HTR-8/SVneo cells, while certain Sch B can attenuate the cytotoxicity.

Key words: Benzo(a)pyrene, Schizandrin, Schizandrin B, Trophoblast, Trophoblast cell, Colorimetry