国际妇产科学杂志

国际妇产科学杂志

• 论著 •    

iNOS和caspase 3在胚胎停育绒毛和蜕膜组织中的表达

郑金艳，连立芬，侯海燕，梁婧，陈俊，陈亚琼，董渠龙   

  1. 300400  天津市北辰医院（郑金艳）；武警后勤学院附属医院妇产科（郑金艳，侯海燕，陈俊，陈亚琼，董渠龙）；秦皇岛市妇幼保健院（连立芬）；中国中医科学院广安门医院南区（梁婧）
  • 出版日期:2017-06-15 发布日期:2017-06-15
  • 通讯作者: 陈亚琼，E-mail：chenyq82@hotmail.com
  • 基金资助:
    国家自然科学基金资助项目（81402691，81528020）；天津市自然科学基金资助项目（15JCQNJC12300，15JCZDJC36000）

Expressions of iNOS and Caspase 3 in Chorionic Tissues and Decidual Tissues of Patients with Embryonic Diapauses

ZHENG Jin-yan, LIAN Li-fen, HOU Hai-yan, LIANG Jing, CHEN Jun, CHEN Ya-qiong, DONG Qu-long   

  1. Tianjin Beichen Hospital, Tianjin 300400, China（ZHENG Jin-yan）；Department of Obstetrics and Gynecology, Affiliated Hospital of Logistics University of the Chinese People′s Armed Forces, Tianjin 300162, Chinａ（ZHENG Jin-yan, HOU Hai-yan, CHEN Jun, CHEN Ya-qiong, DONG Qu-long）；Maternity and Child Care Hospital of Qinhuangdao, Qinhuangdao 066000, Chinａ（LIAN Li-feｎ）；South of Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 102618, Chinａ（ＬＩＡNG Jinｇ）
  • Published:2017-06-15 Online:2017-06-15
  • Contact: CHEN Ya-qiong, E-mail：chenyq82@hotmail.com

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摘要： 目的：探讨胚胎停育绒毛组织和蜕膜组织中半胱氨酸天冬氨酸蛋白酶3（caspase 3）和诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）的表达和临床意义。方法：选择2013年3月—2014年5月就诊于武警后勤学院附属医院妇产科计划生育门诊的30例不明原因早期胚胎停育的患者（病例组）和30例正常早期妊娠妇女（对照组）为研究对象，采用逆转录聚合酶链反应（RT-PCR）方法检测2组绒毛组织和蜕膜组织中caspase 3和iNOS mRNA的表达水平，采用免疫印迹法检测2组绒毛组织和蜕膜组织中caspase 3蛋白的表达水平。结果：①病例组绒毛组织和蜕膜组织中caspase 3 mRNA、总的caspase 3蛋白和活化的caspase 3蛋白的表达水平均高于对照组（P＜0.01）。②病例组绒毛组织和蜕膜组织中iNOS mRNA表达水平低于对照组（P＜0.01）。结论：正常早孕和胚胎停育患者绒毛组织和蜕膜组织中均有iNOS和caspase 3的表达，iNOS和caspase 3可能参与凋亡的增加进而引起胚胎停育的发生。
   

关键词: 胚胎停育, 一氧化氮合成酶, 半胱氨酸天冬氨酸蛋白酶3, 细胞凋亡

Abstract: Objective：To study the expression and the clinical importance of the iNOS and caspase 3 in the chorionic and decidual tissues of the patients whose embryos stop developing. Methods：30 cases of early unexplained embryonic diapauses patients (case group) and 30 normal early pregnant women (control group) for the study, chorionic and deciduas tissues caspase 3 and iNOS levels were tested by RT-PCR method, chorionic and decidual caspase 3 protein expression levels were tested by Western blot. Results：①In the chorionic and decidual tissues, caspase 3 mRNA, the total caspase 3 protein and caspase 3 fragment protein expression levels in the case group was significantly higher than the control group（P＜0.01）. ② In the chorionic and decidual tissues, iNOS mRNA expression level in the case group was significantly lower than the control group（P＜0.01）. Conclusions：In the chorionic and decidual tissues，iNOS and caspase 3 positive expression in the normal pregnancy and in the patients whose embryos stop developing. The iNOS and caspase 3 may involve in apoptosis, and then cause embryo stopping development.

Key words:  Embryonic diapauses, Nitric oxide synthase, Caspase 3, Apoptosis