国际妇产科学杂志

国际妇产科学杂志 ›› 2020, Vol. 47 ›› Issue (5): 550-553.

• 妇科肿瘤研究 论著 • 上一篇    下一篇

迷迭香酸对宫颈癌HeLa细胞凋亡、迁移的影响及与自噬的关系

唐阳芳，陈蕊，廖立冬，王亚玲   

  1. 710077  西安医学院第一附属医院妇科 
  • 收稿日期:2020-05-13 修回日期:2020-07-20 出版日期:2020-10-15 发布日期:2020-10-27
  • 通讯作者: 唐阳芳，E-mail: 68626817@qq.com E-mail:68626817@qq.com
  • 基金资助:
    西安市科技计划项目[2019115213YX007SF040（7）]

Effect of Rosmarinic Acid on Apoptosis and Migration of HeLa Cells in Cervical Cancer and Its Relationship with Autophagy

TANG Yang-fang, CHEN Rui, LIAO Li-dong, WANG Ya-ling   

  1. Department of Gynecology, The First Affiliated Hospital of Xi′an Medical University, Xi′an 710077, China
  • Received:2020-05-13 Revised:2020-07-20 Published:2020-10-15 Online:2020-10-27
  • Contact: TANG Yang-fang, E-mail: 68626817@qq.com E-mail:68626817@qq.com

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摘要： 目的：探讨迷迭香酸对人宫颈癌HeLa细胞凋亡及迁移的影响，并探讨自噬在其中的作用。方法：以不同浓度迷迭香酸（0、20、40、80 μmol/L）处理HeLa细胞48 h，采用流式细胞术检测细胞凋亡，Transwell检测细胞迁移，蛋白质印迹（Western blotting）法检测细胞自噬标志蛋白LC3及Beclin-1的表达。收集宫颈癌组织及正常宫颈组织标本，免疫组织化学法检测组织中LC3及Beclin-1的表达。结果：不同浓度迷迭香酸可诱导HeLa细胞凋亡（F=241.70，P＜0.05），抑制细胞迁移（F=77.63，P＜0.05），并上调LC3及Beclin-1的蛋白表达（LC3：F=240.83，P＜0.05；Beclin-1：F=154.72，P＜0.05），且以上作用随迷迭香酸浓度的增加而增强。宫颈癌组织中LC3及Beclin-1的表达明显低于正常组织，差异有统计学意义（LC3：t=28.00，P＜0.05；Beclin-1：t=19.99，P＜0.05）。结论：自噬失调与宫颈癌的发生有关，迷迭香酸可上调宫颈癌HeLa细胞自噬，并诱导细胞凋亡、抑制细胞迁移，提示迷迭香酸对HeLa细胞的效应可能与其调节自噬有关。

关键词: 迷迭香酸；, 桂皮酸；, 宫颈肿瘤；, 自噬；, HeLa细胞；, 细胞凋亡；, 细胞运动

Abstract: Objective: To investigate the effect of rosmarinic acid on the apoptosis and migration of HeLa cells in human cervical cancer, and the role of autophagy. Methods: HeLa cells were treated with different concentrations of rosmarinic acid (0, 20, 40, 80 μmol/L) for 48 h. Cell apoptosis was detected by flow cytometry and migration was detected by transwell. The expression of autophagy marker proteins LC3 and Beclin-1 was detected by Western blotting. Cervical cancer and normal cervical samples were collected and the expression of LC3 and Beclin-1 in the tissues was detected by immunohistochemistry. Results: Different concentrations of rosmarinic acid could induce HeLa cell apoptosis (F=241.70, P＜0.05), inhibit cell migration (F=77.63, P＜0.05), and up-regulate the expression of LC3 and Beclin-1 (LC3: F=240.83, P＜0.05; Beclin-1: F=154.72, P＜0.05). The above effects of rosmarinic acid were enhanced with the increase of its concentration. The expression of LC3 and Beclin-1 in cervical cancer tissues was significantly lower than that in the control group , and the difference was statistically significant (LC3: t=28.00, P＜0.05; Beclin-1: t=19.99, P＜0.05). Conclusions: Autophagy disorder is related to the occurrence of cervical cancer. Rosmarinic acid can up-regulate the autophagy of HeLa cells of cervical cancer, induce apoptosis and inhibit cell migration, suggesting that the effect of rosmarinic acid on HeLa cells may be related to its regulation of autophagy.

Key words: Rosmarinic acid, Cinnamic acid, Uterine cervical neoplasms, Autophagy, HeLa cells;, Apoptosis;, Cell movement