甲状腺素转运蛋白对卵巢癌细胞株的影响及机制研究

doi:10.12280/gjfckx.20210493

摘要/Abstract

摘要：

目的：探讨甲状腺素转运蛋白（transthyretin,TTR）对卵巢癌细胞株SKOV-3及OVCAR-3迁移和侵袭的影响及其机制。方法：用Lipofectamine 2000搭载转染TTR小干扰RNA（siRNA）或non-target siRNA（对照组）入细胞,采用划痕实验检测细胞迁移能力,Transwell法检测细胞侵袭能力,蛋白质印迹法（Western blotting）检测TTR下游蛋白表达。结果：划痕实验显示,TTR siRNA组和对照组SKOV-3细胞伤口愈合率分为（57.00±5.03）%和（87.33±1.20）%（P=0.004）。TTR siRNA组和对照组OVCAR-3细胞伤口愈合率分为（64.67±5.55）%和（84.33±1.45）%（P=0.027）。Transwell实验显示,TTR siRNA组SKOV-3及OVCAR-3细胞小室穿透的细胞数分别是对照组的（61.00±4.16）%（P=0.013）及（46.33±8.37）%（P=0.003）。Western blotting实验结果显示,在卵巢癌SKOV-3及OVCAR-3细胞,TTR siRNA组Wnt1、β连环素（β-catenin）、基质金属蛋白酶2（MMP-2）和MMP-9的表达都低于对照组（均P<0.05）。结论：TTR可能通过抑制Wnt1/β-catenin通路及其下游关键分子MMP-2和MMP-9的表达,抑制卵巢癌细胞侵袭和迁移。

关键词: 前白蛋白, 卵巢肿瘤, 细胞运动, 肿瘤侵润, Wnt1蛋白质, β连环素, 基质金属蛋白酶9, 基质金属蛋白酶2

Abstract:

Objective：To investigate the effect of transthyretin (TTR) on migration and invasion in ovarian cancer cells and its mechanism. Methods：Lipofectamine 2000 was used to transfect TTR small interfering RNA (siRNA) or non-target siRNA (control group) into cells. Scratch test was used to detect the ability of cell migration, Transwell method was used to detect the ability of cell invasion, the downstream protein expression of TTR was detected by Western blotting. Results：The scratch test showed that the wound healing rate of SKOV-3 cells in the TTR siRNA group and control group was (57.00±5.03)% and (87.33±1.20)% separately (P=0.004). The wound healing rate of OVCAR-3 cells in the TTR siRNA group and control group was (64.67±5.55)% and (84.33±1.45)% separately (P=0.027). Transwell experiments showed that the number of cells penetrated through the chamber in the TTR siRNA group of SKOV-3 and OVCAR-3 cells was (61.00±4.16)% (P=0.013) and (46.33±8.37)% (P=0.003) of the control group, respectively. After suppressing the expression of TTR, Western blotting results showed that the protein expression of Wnt1, β-catenin, MMP-2, and MMP-9 were decreased (P<0.05). Conclusions：TTR may inhibit the invasion and migration of ovarian cancer cells by inhibiting the expression of Wnt1/β-catenin pathway and MMP-2 and MMP-9.

Key words: Prealbumin, Ovarian neoplasms, Cell movement, Neoplasm invasiveness, Wnt1 protein, Beta catenin, Matrix metalloproteinase 9, Matrix metalloproteinase 2

李婧, 刘昀昀, 周晖, 林仲秋, 卢淮武. 甲状腺素转运蛋白对卵巢癌细胞株的影响及机制研究[J]. 国际妇产科学杂志, 2022, 49(1): 39-42.

LI Jing, LIU Yun-yun, ZHOU Hui, LIN Zhong-qiu, LU Huai-wu. The Effect of Transthyretin on Ovarian Cancer Cells and Its Mechanism[J]. Journal of International Obstetrics and Gynecology, 2022, 49(1): 39-42.

图/表 4

图1 SKOV-3细胞株转染TTR siRNA及non-target siRNA序列后划痕实验结果

图2 OVCAR-3细胞株转染TTR siRNA或non-target siRNA序列后划痕实验结果

图3 转染TTR siRNA后对细胞侵袭的影响

图4 SKOV-3及OVCAR-3细胞转染TTR siRNA或non-target siRNA序列后Western blotting实验结果

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