国际妇产科学杂志 ›› 2012, Vol. 39 ›› Issue (2): 199-201.

• 论著 • 上一篇    下一篇

华蟾素与子宫内膜癌HHUA细胞侵袭性生长的实验研究


王绍光, 姜学强
  

  1. 264000 山东省烟台市,青岛大学医学院附属烟台毓璜顶医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-04-15 发布日期:2012-04-15

Effect of Cinobufagin on the Invasive Growth of Endometrial Carcinoma HHUA Cells

WANG Shao-guang,JIANG Xue-qiang   

  1. Department of Obstetrics and Gynecology,Yantai Yuhuangding Hospital,Medical College of Qingdao University,Yantai 264000,Shandong Province,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-04-15 Online:2012-04-15

摘要: 目的:探讨华蟾素对体外培养的子宫内膜癌HHUA细胞侵袭性生长的影响和意义。方法:体外培养内膜癌HHUA细胞,以0.25、2.5和25 g/L的华蟾素干预后,四唑盐比色试验检测细胞增殖,流式细胞仪检测细胞凋亡,反转录-聚合酶链反应检测CD44s表达,transwell小室分析细胞的体外侵袭力。结果:华蟾素对内膜癌HHUA细胞的半数抑制浓度(IC50)为华蟾素生药(0.79±0.21) g/L。对照组CD44s mRNA表达为1.31±0.25、侵袭细胞数为60.50±2.52;0.25 g/L华蟾素对内膜癌HHUA细胞CD44smRNA表达和侵袭力无影响(均P>0.05);2.5 g/L华蟾素干预后,内膜癌HHUA细胞CD44s mRNA表达为1.02±0.23、侵袭细胞数为30.50±3.95,与对照组比较差异有统计学意义(均P<0.05);25 g/L华蟾素干预后,内膜癌HHUA细胞CD44s mRNA表达为0.97±0.39、侵袭细胞数为22.50±4.22,与对照组比较差异有统计学意义(均P<0.05);25 g/L和2.5 g/L华蟾素2个浓度组差异无统计学意义(均P>0.05)。结论:2.5 g/L华蟾素可抑制内膜癌HHUA细胞的侵袭性生长。

关键词: 华蟾蜍毒素, 子宫内膜肿瘤, 基因, 细胞凋亡, 治疗

Abstract: Objective:To investigate the effect of cinobufagin on the invasive growth of the endometrial carcinoma HHUA cells cultured in vitro. Methods:Endometrial carcinoma HHUA cells were cultured in vitro;after the action of cinobufagin with different concentration,MTT method was used to detect the proliferation of HHUA cells,the cellular apoptosis was tested by flow cytometry,the expression of CD44s was measured by RT-PCR,the ability of invasion was compared with transwell chamber model. Results:Endometrial carcinoma HHUA cells expressed CD44s mRNA of (1.31±0.25),number of invasive cell was 60.50±2.52;0.25 g/L cinobufagin didn't affect CD44s and invasive cell(P>0.05); after the action of 2.5 g/L cinobufagin,the expression of CD44s was 1.02±0.23, number of invasive cell was(30.50±3.95),these deta were different from the control group of endometrial carcinoma HHUA cells significantly(P<0.05);after the action of 25 g/L cinobufagin,the expression of CD44s was 0.97±0.39,number of invasive cell was 22.50±4.22,these data were different from the control group of endometrial carcinoma HHUA cells significantly(P<0.05);the action of 25 g/L cinobufagin was similar to that of 0.25 g/L cinobufagin(P>0.05). Conclusions:2.5 g/L cinobufagin inhibit the expression of CD44s mRNA and inhibit the invasive growth of the endometrial carcinoma HHUA cells.

Key words: Cinobufagin, Endometrial neoplasms, Genes, Apoptosis, Therapy