RNA Sequencing of Villus Tissue Reveals Differentially Expressed Genes in Unexplained Recurrent Spontaneous Abortion

doi:10.12280/gjfckx.20230584

Abstract

Abstract:

Objective: To discover the differentially expressed genes (DEGs) in villus tissue of patients with unexplained recurrent spontaneous abortion (URSA) and to analyze their functions, aiming to elucidate the mechanisms behind the onset and progression of URSA. Methods: Villus tissue samples were collected from four URSA patients and four elective termination of pregnancy(ETP) at Department of Gynecology Outpatient of the First Affiliated Hospital of Xinjiang Medical University in March 2022. RNA-seq was employed to screen for DEGs between the two groups, followed by Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to identify the biological functions and pathways of the identified DEGs. A protein-protein interaction (PPI) network was constructed using the STRING database, and screen out the key genes of URSA. Results: RNA-seq analysis identified a total of 3 350 DEGs, of which 2 259 were upregulated genes and 1 091 were downregulated genes in the URSA group. Through PPI network analysis, the top 10 key genes related to URSA were identified, including TYROBP, CD74, GH1, GH2, STAT5A, HLA-DRA, STAT5B, HLA-DRB1, CISH, and HLA-A. Through GO and KEGG biological functional analysis, it was found that differentially expressed mRNA is involved in various immune response pathways related to white blood cell differentiation and allogeneic immune responses. Conclusions: This study utilized transcriptome sequencing technology to obtain the mRNA differential expression profile of URSA, and screened for key genes and biological pathways that may be related to URSA. Our data provide a theoretical basis for elucidating the pathogenesis and diagnostic and therapeutic targests of URSA.

Key words: Abortion, habitual, Villus tissue, RNA, messenger, RNA-Seq, Immunomodulation

YANG Li, YANG Jing, Yeerdengqieqieke , HAN Rui, LA Xiao-lin. RNA Sequencing of Villus Tissue Reveals Differentially Expressed Genes in Unexplained Recurrent Spontaneous Abortion[J]. Journal of International Obstetrics and Gynecology, 2024, 51(3): 322-328.

Figures/Tables 7

References 20

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组别	n	年龄（岁）	孕前BMI （kg/m²）	月经周期（d）	孕龄（d）
试验组	4	30.5±3.4	21.0±1.3	30.3±2.1	54.8±1.7
对照组	4	30.0±3.7	21.0±1.0	29.3±1.5	52.5±1.7
t		0.20	-0.03	0.46	1.85
P		0.85	0.98	0.78	0.11

组别	n	年龄（岁）	孕前BMI （kg/m²）	月经周期（d）	孕龄（d）
试验组	4	30.5±3.4	21.0±1.3	30.3±2.1	54.8±1.7
对照组	4	30.0±3.7	21.0±1.0	29.3±1.5	52.5±1.7
t		0.20	-0.03	0.46	1.85
P		0.85	0.98	0.78	0.11

基因名称	log₂(FC)	URSA_2	URSA_3	URSA_4	URSA_5	ETP_1	ETP_3	ETP_4	ETP_5
TYROBP	2.920 470	17.19	39.88	28.09	20.91	0.75	0.20	1.27	13.30
CD74	5.277 949	12.56	19.26	20.93	4.10	0.21	0.09	0.59	0.70
GH1	3.259 979	35.81	43.56	27.80	92.55	8.50	3.62	4.54	7.11
GH2	2.337 459	702.77	562.54	921.77	1 211.00	333.29	89.32	139.94	186.22
STAT5A	2.498 606	5.22	4.99	6.44	7.24	1.05	0.81	1.23	1.73
HLA-DRA	6.050 772	51.18	65.28	82.84	16.28	0.21	0.12	2.25	1.02
STAT5B	1.442 519	19.52	20.66	21.93	29.65	11.81	6.84	8.98	10.40
HLA-DRB1	3.704 165	11.65	11.18	17.43	4.68	1.26	0.06	0.90	1.47
CISH	1.900 941	5.17	6.94	6.67	9.41	2.83	1.46	1.50	2.64
HLA-A	2.658 422	42.37	62.90	53.30	16.44	3.34	10.97	4.26	12.22

基因名称	log₂(FC)	URSA_2	URSA_3	URSA_4	URSA_5	ETP_1	ETP_3	ETP_4	ETP_5
TYROBP	2.920 470	17.19	39.88	28.09	20.91	0.75	0.20	1.27	13.30
CD74	5.277 949	12.56	19.26	20.93	4.10	0.21	0.09	0.59	0.70
GH1	3.259 979	35.81	43.56	27.80	92.55	8.50	3.62	4.54	7.11
GH2	2.337 459	702.77	562.54	921.77	1 211.00	333.29	89.32	139.94	186.22
STAT5A	2.498 606	5.22	4.99	6.44	7.24	1.05	0.81	1.23	1.73
HLA-DRA	6.050 772	51.18	65.28	82.84	16.28	0.21	0.12	2.25	1.02
STAT5B	1.442 519	19.52	20.66	21.93	29.65	11.81	6.84	8.98	10.40
HLA-DRB1	3.704 165	11.65	11.18	17.43	4.68	1.26	0.06	0.90	1.47
CISH	1.900 941	5.17	6.94	6.67	9.41	2.83	1.46	1.50	2.64
HLA-A	2.658 422	42.37	62.90	53.30	16.44	3.34	10.97	4.26	12.22