Abstract: Objective：Statistics in the distribution of data in all types of HPV and CINⅡ+ cases of cervical cancer in the distribution of data for HPV vaccine introduction in China and to provide baseline clinical trials for evaluation of the four HPV vaccine. Methods：SPF10-PCR，DEIA and LiPA technology were used to detect the type of HPV from paraffin-embedded cervical specimens. Results：It showed HPV positive in all the 91 paraffin-embedded cervical specimens by DEIA．And there are 89 postive cases by LiPA，among which 72 cases are single HPV type，17 cases are mixed HPV types. The top three prevalence of HPV types are follow：16，58，18. Conclusions：In Henan the most common HPV subtype is HPV 16 （80.9%），the second is HPV 58（10.1%）， the current preventive vaccine has been developed to carry out the crowd in our vaccine provides a theoretical basis for intervention，while 58 was the importance of the study population for novel vaccine development challenges.

Key words: Human papillomavirus 16, Papillomavirus vaccines, Uterine cervical neoplasms, Polymerase chain reaction, Cervical intraepithelial neoplasia