Abstract: Objective： To detect TNF-α，E2，E6 and E7 in different cervical lesion and analyse the relationship between them. Methods：According to different pathology result， specimens were divided into normal group of HPV negative，HPV16 positive normal group，HPV16 positive CINⅠ-Ⅱ，HPV16 positive CINⅢ，HPV16 positive SCC group. TNF-α，E2，E6 and E7 proteins of each group specimens were detected by using RT-PCR，and the result of the test were analyzed statistically. Results：HPV E6 and E7 gene expression is positively correlated with the malignant degree of cervical cells，and the E7 gradually rising trend is more significant. The E2 fall after rise to CINⅡ，and of TNF-α basic is the rising trend in different cervical lesions ，but the expression has no obvious difference between CINⅢ group and cervical invasive cancer. Conclusions：With the ratio of E2/E7 to predict or diagnose the degree of high cervical lesions might be more accurate. Elevated inflammatory cytokines of TNF-α may increase the destruction of E2, which lead to excessive expression of oncogene E6 and E7，thus promote the occurrence of cervical cancer.

Key words: Uterine cervical neoplasms, Cervical intraepithelial neoplasia, Tumor necrosis factor-alpha, Reverse transcriptase polymerase chain reaction, Papillomaviridae