Study on the Influence of Cervicitis Status on HPV Infection

doi:10.12280/gjfckx.20230750

Abstract

Abstract:

Objective: To investigate the effect of cervicitis status on human papilloma virus (HPV) infection. Methods: The E6-c-Myc-P2A-E7-c-3Flag gene lentivirus was used to infect the control group (untreated human normal cervical epithelial cells) and the experimental group [human normal cervical epithelial cells induced with lipopolysaccharide (1 μg/mL) for 24 h], respectively, and observe the effect of cervicitis status on the expression of Myc-E6 and Flag-E7 tagged proteins. Results: Compared with the control group, the expression of human interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), IL-1β, and IL-17A mRNA was significantly increased in the experimental group, and the differences were statistically significant (both P<0.05). After 72 h of infection with the E6-c-Myc-P2A-E7-c-3Flag gene lentivirus, the transfection efficiency of the experimental group and the control group was (89.05±0.16)% and (79.30±1.97)%, respectively, with a statistically significant difference (P<0.05). The expression of Myc-E6 and Flag-E7 tagged proteins in the experimental group was significantly increased compared to the control group, and the difference was statistically significant (both P<0.05). Conclusions: Cervical epithelial cells are more susceptible to HPV infection when they are in an inflammatory state.

Key words: Uterine cervicitis, Papillomavirus infections, Lipopolysaccharides, Lentivirus infections, Interleukins, Tumor necrosis factor-alpha

WANG Qiang, SONG Dian-rong. Study on the Influence of Cervicitis Status on HPV Infection[J]. Journal of International Obstetrics and Gynecology, 2023, 50(6): 627-631.

Figures/Tables 6

References 15

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基因	引物序列
IL-17A	正向引物：5′CAGGAGCGACCCAGAAAGTT3′
	反向引物：5′GCAGTAACTGTTGCTTGCAGT3′
TNF-α	正向引物：5′AGGTCCGAAAACACTGTGAGT3′
	反向引物：5′AGCAAGCGGTTCTTCCCTTC3′
IL-6	正向引物：5′ATGATGGCTTATTACAGTGGCAA3′
	反向引物：5′GTCGGAGATTCGTAGCTGGA3′
IL-1β	正向引物：5′ACTCACCTCTTCAGAACGAATTG3′
	反向引物：5′CCATCTTTGGAAGGTTCAGGTTG3′

基因	引物序列
IL-17A	正向引物：5′CAGGAGCGACCCAGAAAGTT3′
	反向引物：5′GCAGTAACTGTTGCTTGCAGT3′
TNF-α	正向引物：5′AGGTCCGAAAACACTGTGAGT3′
	反向引物：5′AGCAAGCGGTTCTTCCCTTC3′
IL-6	正向引物：5′ATGATGGCTTATTACAGTGGCAA3′
	反向引物：5′GTCGGAGATTCGTAGCTGGA3′
IL-1β	正向引物：5′ACTCACCTCTTCAGAACGAATTG3′
	反向引物：5′CCATCTTTGGAAGGTTCAGGTTG3′

组别	n	IL-6	TNF-α	IL-1β	IL-17A
对照组	3	1.03±0.28	1.01±0.19	1.00±0.11	1.00±0.01
实验组	3	4.07±0.00	5.70±0.36	4.91±0.43	6.25±0.31
t		18.480	19.980	15.840	29.400
P		<0.001	<0.001	<0.001	<0.001

组别	n	IL-6	TNF-α	IL-1β	IL-17A
对照组	3	1.03±0.28	1.01±0.19	1.00±0.11	1.00±0.01
实验组	3	4.07±0.00	5.70±0.36	4.91±0.43	6.25±0.31
t		18.480	19.980	15.840	29.400
P		<0.001	<0.001	<0.001	<0.001

组别	n	Myc-E6	Flag-E7
对照组	3	0.78±0.01	0.66±0.05
实验组	3	1.07±0.01	1.06±0.01
t		65.470	63.030
P		<0.001	<0.001