基于PI3K/Akt信号通路探讨中药单体调控宫颈癌HeLa细胞研究进展

doi:10.12280/gjfckx.20250391

摘要/Abstract

摘要：

宫颈癌是最常见的妇科恶性肿瘤之一，磷脂酰肌醇3激酶（phosphoinositide 3-kinase，PI3K）/蛋白激酶B（protein kinase B，Akt）信号通路是与宫颈癌关系密切的信号转导通路之一。研究发现，槲皮素、木犀草素、桦褐孔菌三萜、土贝母苷甲及甲基莲心碱可抑制PI3K/Akt通路激活，降低PI3K和Akt磷酸化水平，诱导HeLa细胞凋亡；汉黄芩素、鱼藤素、柴胡皂A、石蒜碱、喜树碱、紫草素及青蒿素通过该通路激活促凋亡蛋白及自噬复合物，促进细胞自噬与凋亡；丹皮酚、氧化苦参碱及原人参二醇则通过调控PI3K/Akt信号通路介导的上皮-间质转化，抑制细胞迁移和侵袭；三氧化二砷、紫杉醇及新藤黄酸可阻断该通路，降低血管生成相关蛋白表达，抑制肿瘤血管新生；紫檀芪通过该通路下调哺乳动物雷帕霉素靶蛋白表达，影响肿瘤微环境。中药单体通过调控PI3K/Akt信号通路，影响HeLa细胞的增殖、凋亡、自噬、迁移、侵袭及肿瘤微环境，为宫颈癌提供了新的治疗视野和理论依据。

关键词: 宫颈肿瘤, HeLa细胞, 磷酸肌醇3-激酶类, 蛋白质丝氨酸苏氨酸激酶, 信号传导

Abstract:

Cervical cancer is one of the most common gynecological malignancies. The phosphoinositide 3-kinase (PI3K) / protein kinase B (Akt) signaling pathway is one of the signal transduction pathways closely related to cervical cancer. Studies have found that quercetin, luteolin, triterpenoids from Inonotus obliquus, tubeimoside Ⅰ, and neferine can inhibit the activation of the PI3K/Akt pathway, reduce the phosphorylation levels of PI3K and Akt, and induce apoptosis in HeLa cells. Wogonin, rotenone, saikosaponin A, lycorine, camptothecin, shikonin, and artemisinin activate pro-apoptotic proteins and autophagic complexes through this pathway, promoting cell autophagy and apoptosis. Paeonol, oxymatrine, and protopanaxadiol inhibit cell migration and invasion by regulating the epithelial-mesenchymal transition mediated by the PI3K/Akt signaling pathway. Arsenic trioxide, paclitaxel, and gambogic acid can block this pathway, reduce the expression of angiogenesis-related proteins, and inhibit tumor angiogenesis. Pterostilbene down-regulates the expression of mammalian target of rapamycin through this pathway, affecting the tumor microenvironment. Traditional Chinese medicine monomers affect the proliferation, apoptosis, autophagy, migration, invasion of HeLa cells and the tumor microenvironment by regulating the PI3K/Akt signaling pathway, providing new therapeutic perspectives and theoretical basis for cervical cancer.

Key words: Uterine cervical neoplasms, HeLa cells, Phosphatidylinositol 3-kinases, Protein-serine-threonine kinases, Signal transduction

尚晶莹, 刘彤彤, 谷栩萌, 王嘉萌, 丛慧芳. 基于PI3K/Akt信号通路探讨中药单体调控宫颈癌HeLa细胞研究进展[J]. 国际妇产科学杂志, 2025, 52(5): 498-504.

SHANG Jing-ying, LIU Tong-tong, GU Xu-meng, WANG Jia-meng, CONG Hui-fang. Research Progress on the Regulation of Cervical Cancer HeLa Cells by Traditional Chinese Medicine Monomers Based on the PI3K/Akt Signaling Pathway[J]. Journal of International Obstetrics and Gynecology, 2025, 52(5): 498-504.

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