国际妇产科学杂志

国际妇产科学杂志 ›› 2011, Vol. 38 ›› Issue (3): 211-213.

• 论著 • 上一篇    下一篇

胎盘绒毛组织内质网蛋白44表达与子痫前期的关系

孙丽洲,姜子燕,任怀彬   

  1. 210029 江苏省南京市，南京医科大学第一附属医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-06-15 发布日期:2011-06-15
  • 通讯作者: 孙丽洲

Relationship Between the Expression of Endoplasmic Reticulum Protein （ERp44） and Pre-eclampsia in Placenta

SUN Li-zhou，JIANG Zi-yan，REN Huai-bin   

  1. Department of Obstetrics and Gynaecology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2011-06-15 Online:2011-06-15
  • Contact: SUN Li-zhou

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摘要： 目的：探讨子痫前期胎盘绒毛组织内质网蛋白44（ERp44）表达与子痫前期发病的关系。方法：采用免疫组化法、半定量反转录聚合酶链反应（RT-PCR）及蛋白质印迹杂交法检测子痫前期及正常胎盘绒毛组织（各20例）中ERp44基因及蛋白表达情况。结果：免疫组化结果显示，20例子痫前期胎盘绒毛组织均存在ERp44染色阳性，而正常组阴性染色；子痫前期胎盘绒毛组织ERp44 mRNA的表达水平与正常组差异无统计学意义（P ＞0.05）；而子痫前期胎盘绒毛组织ERp44 蛋白表达水平较正常组明显升高（P＜0.05）。结论：ERp44表达可能与子痫前期发病机制密切相关。

关键词: 蛋白质类, 先兆子痫, 胎盘, 内质网, 免疫组织化学, 印迹法, 蛋白质

Abstract: Objective：This study was designed to investigate the relationship between endoplasmic reticulum protein（ERp44） expression and the pathogenesis of preeclampsia in preeclampsia placental villi. Methods：Immunohistochemistry, semi-quantitative RT-PCR and Western blot were used to detect the ERp44 gene and protein expression in preeclampsia and normal placental villi（both 20 cases）. Results：Immunohistochemistry showed that ERp44 staining were strongly positive in all the 20 pre-eclampsia placental villi, while the normal group showed negative staining；RT-PCR showed no significant difference in ERp44 mRNA in two groups（P＞0.05）；Western blot assay found that the ERp44 protein level in pre-eclampsia villi was significantly higher than normal group（P＜0.05）. Conclusions：The expression of ERp44 may be closely related to the pathogenesis of pre-eclampsia.

Key words: Proteins, Pre-eclampsia, Placenta, Endoplasmic reticulum, Immunohistochemistry, Blotting