国际妇产科学杂志

国际妇产科学杂志 ›› 2012, Vol. 39 ›› Issue (5): 533-534.

• 论著 • 上一篇    下一篇

四甲基偶氮唑蓝法测定植酸酮对HeLa细胞的作用


武志丹, 杨志谋, 岳天孚   

  1. 300071 天津,南开大学药学院(武志丹),生命科学学院(杨志谋);天津医科大学总医院(岳天孚)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-10-15 发布日期:2012-10-15

Experimental Study of Mefordcare Phytic Acid Ketone Effect on HeLa Cells Using MTT Assay

WU Zhi-dan，YANG Zhi-mou，YUE Tian-fu   

  1. College of Pharmacy，Nankai University，Tianjin 300071，China（WU Zhi-dan）；College of LifeSciences，Nankai University，Tianjin 300071，China（YANG Zhi-mou）；Tianjin Medical University General Hospital，Tianjin 300052，China（YUE Tian-fu）
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-10-15 Online:2012-10-15

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摘要： 目的：分析植酸酮妇科清洗装置（商品名：美福康）Ⅱ型1号及2号对体外培养的HeLa细胞生长的抑制作用，为后期实验提供实验依据。方法：体外培养HeLa细胞，分为实验组和对照组，实验组从8个浓度水平上给药，分别经过24，48，72 h后用四甲基偶氮唑蓝（MTT）法检测药物对HeLa细胞增殖的影响。结果：与对照组相比，经2种型号的药物作用后的HeLa细胞存活率降低，凋亡增加，作用呈时间-剂量依赖关系。结论：植酸酮妇科清洗装置Ⅱ型1号及2号均能有效抑制宫颈癌HeLa细胞的生长。

关键词: HeLa细胞, 宫颈肿瘤, 乳头状瘤病毒科, 植酸酮, 四甲基偶氮唑蓝

Abstract: Objective：To study the ability of the No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone on
inhibiting the proliferation of HeLa cells which were cultivated in vitro，and provide the evidence for futher experiment. Methods：The HeLa cells which were cultivated in vitro was treated by eight different concentrations for 24，48 and 72h，MTT assay was employed to examine the growth suppression of HeLa cells. Results：Contrast with the control group，the apoptosis of the cells treated with these two numbers of the drug was increased and the growth rate was decreased. And the inhibition of HeLa cells in partly is in a concentration-dependent and time-dependent manner. Conclusions：The No.1 and No.2 of type Ⅱ of Mefordcare Phytic Acid Ketone could significantly inhibit the proliferation of HeLa cells.

Key words: HeLa cells, Uterine cervical neoplasms, Papillomaviridae, Phytic acid ketone, Methyl thiazolyl tetrazolium