国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (4): 445-448.

• 论著 • 上一篇    下一篇

TLR3在宫颈HPV16持续性感染及宫颈病变中的作用

唐荣荣，刘佳佳，刘荣，张丽志，罗远材，瞿全新   

  1. 300192 天津市第一中心医院（唐荣荣，刘佳佳，刘荣，张丽志，罗远材，瞿全新）；天津市西青医院（唐荣荣）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-08-15 发布日期:2015-08-15
  • 通讯作者: 瞿全新

Study on the Role of TLR3 in Cervical HPV16 Persistent Infection and Cervical Lesions

TANG Rong-rong，LIU Jia-jia，LIU Rong，ZHANG Li-zhi，LUO Yuan-cai，QU Quan-xin   

  1. Tianjin First Central Hospital，Tianjin 300192，China（TANG Rong-rong, LIU Jia-jia, LIU Rong, ZHANG Li-zhi, LUO Yuan-cai, QU Quan-xin）；Tianjin Xiqing Hospital, Tianjin 300380, China（TANG Rong-rong）
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-08-15 Online:2015-08-15
  • Contact: QU Quan-xin

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摘要： 目的：探讨Toll样受体3（TLR3）在宫颈人乳头瘤病毒16（HPV16）持续性感染及宫颈病变中的作用。方法：选取宫颈液基细胞学检查正常的HPV16阳性者157例，其中40例6个月后复查仍为HPV16阳性（HPV16持续感染组），117例6个月后复查转为阴性（HPV16非持续感染组）。同时选择同期就诊的宫颈HPV16阳性、临床病理资料完整的宫颈疾病患者共206例，其中A组119例（慢性宫颈炎102例、CINⅠ17例）、B组66例（宫颈CINⅡ~Ⅲ 57例，原位癌9例）和C组21例（宫颈浸润癌）。以聚合酶链反应（PCR）方法检测TLR3及β干扰素（IFN-β）DNA相对表达量，应用基因测序方法进行TLR3基因突变分析。结果：HPV16持续感染组TLR3、IFN-β DNA相对表达量低于HPV16非持续感染组（均P＜0.05）。HPV16持续感染组与非持续感染组TLR3与IFN-β DNA相对表达量均呈正相关（均P＜0.001）。在HPV16持续感染组与非持续感染组中均未检测到TLR3扩增片段的基因突变。HPV16阳性宫颈病变的3组患者TLR3及IFN-β DNA相对表达量差异有统计学意义（均P＜0.05）。TLR3、IFN-β DNA相对表达量均为A组高于B组及C组（均P＜0.05），B组高于C组（P＜0.05）。在不同级别宫颈病变中TLR3与IFN-β DNA相对表达量呈正相关（均P＜0.001）。结论：TLR3可能通过调控IFN-β表达而调节宫颈局部免疫功能，TLR3表达减低不仅与宫颈HPV16持续性感染有关，而且在宫颈癌前病变及宫颈癌发生及进展中发挥重要作用。

关键词: 宫颈肿瘤, 人乳头瘤病毒16, 感染, Toll样受体3, 干扰素β

Abstract: Objective：To explore the role of TLR3 in HPV16 persistent infection and cervical lesions. Methods：Collected 157 cases HPV 16 infected patients whose liquid-base cervical cytology report is no intraepithelial lesion or malignancy（NILM）. After 6 months observation, among them, 40 cases were detected HPV16 persistent infection（persistent infection group）, and the other 107 cases turned to be negative （non-persistent infection group）, meanwhile selected 206 cases HPV 16 infected patients and split them with different stages of cervical lesions into three groups :group A contains 119 cases （chronic cervicitis 102 cases and CINⅠ 17 cases）; group B contains 66 cases （CINⅡ-Ⅲ 57 cases and carcinoma in situ 9 cases）; group C contains 21 cases （cervical carcinoma 21 cases）. The expression of DNA TLR3 and IFN-β were detected by PCR. The mutation of TLR3 recycled productions were detected by gene sequencing respectively. Results：The expression levels of TLR3, IFN-β in persistent infection group were lower than those in non-persistent infection group, and the differences were statistically significant（P＜0.05）. TLR3 was positively correlated with IFN-β, which has statistically significant（P＜0.001）. The expressions of TLR3, IFN-β in different cervical lesions groups: in group A, B and C, the expression levels of TLR3 group A was higher than group B and group C （P＜0.05）; the expression levels of group B was higher than group C（P＜0.05）. The expression levels of IFN-β group A was higher than group B and group C （P＜0.05）; the expression levels of group B was higher than group C （P＜0.05). In three groups TLR3 was positively correlated with IFN-β, which has statistically significant（all P＜0.001）. Conclusions：The low expression level of TLR3 leads to cervical local immune dysfunction by down-regulating the expression of IFN-β. The reduction of TLR3 expression is not only related with the cervical HPV 16 persistent infection but also plays an important role in regulating the occurrence and progression of cervical lesions and cervical cancer.

Key words: Uterine cervical neoplasms, Human papillomavirus 16, Infection, Toll-like receptor 3, Interferon-beta