国际妇产科学杂志

国际妇产科学杂志 ›› 2016, Vol. 43 ›› Issue (1): 45-48.

• 论著 • 上一篇    下一篇

五味子乙素对BaP致HTR8-SVneo细胞损伤的保护作用及机制

吴念，陈亚琼，陈晓，侯海燕，王华   

  1. 570203武警海南总队医院妇产科（吴念）；中国人民武装警察部队后勤学院附属医院妇产科（陈亚琼，陈晓，侯海燕，王华）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: 陈亚琼

The Protection Effect and Mechanism of Schisandrin B on Extravillous Trophoblast Cell Line Damage Caused by BaP

WU Nian，CHEN Ya-qiong，CHEN Xiao，HOU Hai-yan，WANG Hua   

  1. Department of Obstetrics and Gynecology，Hainan Provincial Corps Hospital，Chinese People′s Armed Police Forces，Haikou 570203，China（WU Nian）；Department of Obstetrics and Gynecology，Affiliated Hospital，Logistics University of Chinese People′s Armed Police Forces，Tianjin 300162，China（CHEN Ya-qiong，CHEN Xiao，HOU Hai-yan，WANG Hua）
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-02-15 Online:2016-02-15
  • Contact: CHEN Ya-qiong

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摘要： 目的：研究五味子乙素（Schisandrin B，Sch B）预防苯并芘（BaP）致人绒毛膜外滋养层细胞（HTR8-SVneo）损伤的作用机制。方法：通过新型四唑化合物（MTS）法观察和测定，最终选取0.5，1，2 μmol/L Sch B分别作用HTR8-SVneo细胞24 h后再给予20 μmol/L Bap染毒处理24 h，MTS检测各组细胞生长情况；JC-1荧光染色法观察各组线粒体膜电位荧光强度变化；酶联免疫吸附试验（ELISA）检测各组细胞内促凋亡蛋白（Bax）、细胞色素C（cytochromec，cyt-c）蛋白浓度；蛋白质印迹法（Western blotting）检测各组的凋亡蛋白（Caspase-9）。结果：①BaP染毒组OD值为1.187±0.015，0.5，1，2 μmol/L Sch B预保护24 h后的OD值分别为1.483±0.022、1.489±0.048和1.531±0.240，差异有统计学意义（P＜0.05）。②BaP染毒组线粒体荧光强度弱，0.5，1，2 μmol/L Sch B预保护24 h后的荧光强度较强，差异有统计学意义（P＜0.05）。③BaP染毒组Bax、cyt-c蛋白浓度升高，0.5，1，2 μmol/L Sch B预保护24 h后细胞内Bax、cyt-c蛋白浓度降低，差异有统计学意义（P＜0.05）。④BaP染毒组细胞内caspase-9蛋白表达升高，0.5，1，2 μmol/L Sch B预保护24 h后细胞内caspase-9蛋白表达降低，差异有统计学意义（P＜0.05）。结论：Sch B可以预防BaP致HTR8-SVneo细胞损伤，其作用机制可能与抗线粒体途径有关。

关键词: 五味子乙素, 苯并芘, 人绒毛膜外滋养层细胞, 线粒体, 细胞色素C

Abstract: Objective：To explore the protection mechanism of Schisandrin B on extravillous trophoblast cell line damage caused by BaP. Methods：After 0.5，1，2 μmol/L Sch B deal with human HTR8-SVneo cells 24 h，added 20 μmol/L Bap 24 h，using MTS measured cells growth. Used JC-1 fluorescent staining to measure the change of mitochondrial membrane potential in each group. Respectively used ELISA to measure the concentration of Bax and cyt-c protein in each group. Respectively used Western blotting to analyse the expression of caspase-9 in each group. Results：①MTS results showed that BaP alone cell OD was 1.187±0.015，0.5，1，2 μmol/L Sch B the role of OD was 1.483±0.022，1.489±0.048 and 1.531±0.240，compared with BaP alone group，the differences were statistically significant（P＜0.05）. ②JC-1 fluorescent staining results showed that BaP alone cell mitochondrial fluorescent intensity was lower and 0.5，1，2 μmol/L Sch B the role of mitochondrial fluorescent intensity was higer. Compared with BaP alone group，the differences were statistically significant（P＜0.05）. ③ ELISA results showed that BaP alone cell the concentration of Bax，cyt-c protein were higher and 0.5，1，2 μmol/L Sch B the role of the concentration of Bax，cyt-c protein were lower，respectively. Compared with BaP alone group，the differences were statistically significant（P＜0.05）. ④Western blotting results showed that BaP alone cell the concentration of caspase-9 protein was higer，and 0.5，1，2 μmol/L Sch B the role of the concentration of caspase-9 protein was lower，respectively. Compared with BaP alone group，the differences were statistically significant（P＜0.05）. Conclusions：Schisandrin B can prevent damage of extravillous trophoblast cell line caused by BaP，whose mechanism may be associated with resistance to mitochondrial pathway.

Key words: Schisandrin B, BaP, Extravillous trophoblast cell, Mitochondria, Cyt-c