国际妇产科学杂志

国际妇产科学杂志 ›› 2016, Vol. 43 ›› Issue (5): 590-593.

• 论著 • 上一篇    下一篇

五味子乙素对BaP致HTR-8/SVneo侵袭力损伤的保护

王梦，梁婧，侯海燕，董渠龙，陈亚琼   

  1. 300162 天津，中国人民武装警察部队后勤学院附属医院妇产科（王梦，侯海燕，董渠龙，陈亚琼）；天津市职业与环境危害防制重点实验室（侯海燕，陈亚琼）；中国中医科学院广安门医院南区（梁婧）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-10-15 发布日期:2016-10-15
  • 通讯作者: 陈亚琼

The Protection Effect of Schisandrin B on Extravillous Trophoblast Cell Line Aggressive Damage Caused by BaP

WANG Meng，LIANG Jing，HOU Hai-yan，DONG Qu-long，CHEN Ya-qiong   

  1. Department of Obstetrics and Gynecology，Affiliated Hospital of Medical College of Chinese People′s Armed Police Forces，Tianjin 300162，China（WANG Meng，HOU Hai-yan，DONG Qu-long，CHEN Ya-qiong）；Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards，Tianjin 300162，China（HOU Hai-yan，CHEN Ya-qiong）；Guang′anmen Hospital China Academy of Chinese Medical Sciences，Beijing 100053，China（LIANG Jing）
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-10-15 Online:2016-10-15
  • Contact: CHEN Ya-qiong

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摘要： 目的：以人绒毛膜外滋养层细胞（human trophoblast HTR-8/SVneo，简称HTR）细胞株为研究载体，研究五味子乙素（Schisandrin B，Sch B）对苯并芘（benzo[a]pyrene，BaP）致HTR细胞侵袭力损伤的影响。方法：实验分为对照组、BaP染毒组、不同浓度Sch B（0.5，1，2 μmol/L）干预染毒组，通过新型四唑化合物（MTS）法检测细胞增殖情况，明确BaP染毒及Sch B干预剂量；通过Transwell侵袭实验检测BaP染毒后以及Sch B干预后细胞侵袭力的改变。结果：①MTS检测细胞增殖情况：与对照组相比，20 μmol/L浓度BaP作用后细胞增殖明显下降（P＜0.01），不同浓度Sch B（0.5，1，2 μmol/L）干预后细胞增殖情况优于BaP染毒组（P＜0.05）。②Transwell细胞侵袭实验：与对照组相比，BaP染毒组细胞侵袭力明显下降（P＜0.01），不同浓度Sch B干预组细胞侵袭力也呈下降趋势（P＜0.05）；与BaP染毒组相比，Sch B干预组细胞侵袭力明显提升（P＜0.05）。结论：一定浓度的BaP会对HTR的细胞增殖及侵袭力造成损伤，Sch B可以预防BaP对HTR细胞增殖造成的损伤，同时提高细胞侵袭力。

关键词: 五味子属, 苯并芘, 细胞增殖, 滋养层细胞

Abstract: Objective: Using human trophoblast HTR-8/SVneo (HTR) cell lines for the study of the carrier to study Schisandrin B (Sch B)′s action of benzo[a]pyrene (BaP) damage effect on HTR cell lines aggressivity. Methods：Experiment is divided into the control group, the BaP infected group, different dose Sch B (0.5, 1, 2 μmol/L), intervention infected groupcell proliferation by MTS experimental detection, clear BaP canister and Sch B intervention dose, by Transwell invasion experiment after the detection of BaP infected cells and Sch B cell invasion force change after intervention. Results：①The MTS test cell proliferation,compared with control group, 20 μmol/L BaP concentration cell proliferation significantly decreased after effect （P＜0.01）, after Sch B （0.5, 1, 2 μmol/L） intervention the cell proliferation is obviously better than BaP infected group（P＜0.05）. ②Transwell cell invasion experiment, compared with control group, the BaP cells infected group was much less aggressive （P＜0.05）. Sch B intervention group cell invasion force also declined （P＜0.05）. Compared with the infected group, Sch B cells of aggressive intervention group was obviously ascending （Ｐ＜０．０５）. Conclusions：①A certain concentrations of BaP damaged HTR cell proliferation and invasion force. ②Sch B can prevent the damage of BaP of HTR cell proliferation, and at the same time improve cell aggressivity.

Key words: Schisandra, Benzo(a)pyrene, Cell proliferation, Trophoblast cell