国际妇产科学杂志

国际妇产科学杂志 ›› 2019, Vol. 46 ›› Issue (2): 154-158.

• 论著 • 上一篇    下一篇

EMSY基因表达水平和多态性与早期卵巢上皮癌患者预后的关系研究

佟春艳，柳春韬，王学慧，刘丽霞   

  1. 116001 大连大学附属中山医院妇科二病房
  • 收稿日期:2018-08-14 修回日期:2018-12-19 出版日期:2019-04-15 发布日期:2019-04-15
  • 通讯作者: 柳春韬，E-mail：649629247@qq.com E-mail:tong512512@sina.com

Relationship between EMSY Gene Expression and Polymorphism and Prognosis in Patients with Early Stage Ovarian Cancer

TONG Chun-yan，LIU Chun-tao，WANG Xue-hui，LIU Li-xia   

  1. Department of Gynecology，Zhongshan Hospital，Dalian University，Dalian 116001，Liaoning Province，China
  • Received:2018-08-14 Revised:2018-12-19 Published:2019-04-15 Online:2019-04-15
  • Contact: LIU Chun-tao，E-mail：649629247@qq.com E-mail:tong512512@sina.com

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摘要： 目的：探讨EMSY基因表达水平和多态性与早期卵巢上皮癌患者预后的关系。方法：选取118例早期卵巢上皮癌患者（研究组）及63例良性妇科肿瘤患者（对照组），采用逆转录-聚合酶链反应（RT-PCR）分析EMSY mRNA，PCR-限制性片段长度多态性（RFLP）检测421+242A＞G位点多态性，并进一步分析EMSY基因表达水平及多态性与预后的关系。结果：RT-PCR检测显示，研究组EMSY mRNA和乳腺癌易感基因2（BRCA2）mRNA相对表达量高于对照组，差异有统计学意义（t=33.592，P=0.000；t=25.046，P=0.000）。年龄、绝经情况、国际妇产科联盟（FIGO）分期、分化程度、病理类型、术后化疗及术后复发与EMSY mRNA表达水平相关（均P＜0.05）。研究组EMSY基因421+242A＞G位点3种基因型和2种等位基因分布频率与对照组比较差异有统计学意义（均P＜0.05）。EMSY基因421+242A＞G位点GG基因型总生存期、疾病无进展生存期与AA基因型比较差异有统计学意义（Log-rank χ2=7.482，P=0.006；Log-rank χ2=8.406，P=0.004）。EMSY mRNA高表达是影响患者总生存期的危险因素（OR=2.322，95%CI：1.277～5.031，P=0.027），AA基因型是影响患者疾病无进展生存期的危险因素（OR=1.882，95%CI：1.192～4.323，P=0.039）。结论：初步研究证实EMSY基因表达水平及421+242A＞G位点多态性与早期卵巢上皮癌预后有关。

关键词: 卵巢肿瘤, EMSY基因, 多态性, 限制性片段长度, 预后

Abstract: Objective：To investigate the relationship between EMSY gene expression and polymorphism and prognosis in early ovarian epithelial cancer. Methods: 118 cases of early ovarian epithelial cancer (study group) and 63 patients with benign tumor (control group) were selected. EMSY mRNA was analyzed by RT-PCR, 421+242A＞G locus polymorphism was detected by PCR-RFLP, and the relationship between EMSY gene expression level and polymorphism and prognosis was further analyzed. Results: RT-PCR test showed that the relative expression of EMSY mRNA and BRCA2 mRNA of the study group were higher than those of the control group, the difference was statistically significant (t=33.592, P=0.000; t=25.046, P=0.000). Age, menopause, FIGO stage, differentiation degree, pathological type, postoperative chemotherapy and postoperative recurrence were associated with EMSY mRNA expression levels(P＜0.05). The frequency of the three genotypes and the two alleles of the EMSY gene 421+242A＞G locus in the study group were significantly different from those in the control group (P＜0.05), and the total survival rate of GG genotype and the progression free survival rate of 421+242A＞G locus of EMSY gene were statistically significant (Log-rank χ2=7.482, P=0.006; Log-rank χ2=8.406, P=0.004). High expression of EMSY mRNA affects the survival of the patient (OR=2.322, 95%CI: 1.277-5.031, P=0.027), and the AA genotype affects the progression free survival of the patients (OR=1.882, 95%CI: 1.192-4.323, P=0.039). Conclusions: EMSY gene expression and 421+242A＞G polymorphism are related to the prognosis of early ovarian cancer, which can be further studied.

Key words: Ovarian neoplasms, EMSY gene, Polymorphism, restriction fragment length, Prognosis