国际妇产科学杂志

国际妇产科学杂志 ›› 2020, Vol. 47 ›› Issue (2): 232-235.

• 论著 • 上一篇    下一篇

基质细胞衍生因子1、趋化因子受体4和淋巴管新生在子宫内膜异位症中的意义

周雅娟，开海丽，乔海风，张连锁，吴刘成，刘颖蕾，刘曼华   

  1. 226001 江苏省南通市，南通大学第二附属医院妇产科（周雅娟，开海丽，乔海风，张连锁，刘颖蕾，刘曼华）；南通大学实验动物中心（吴刘成）
  • 收稿日期:2019-11-12 修回日期:2020-02-28 出版日期:2020-04-15 发布日期:2020-04-15
  • 通讯作者: 刘颖蕾，E-mail：summerlyl622@163.com；刘曼华，E-mail：liumanhua622@163.com E-mail:liumanhua2010@hotmail.com
  • 基金资助:
    南通市科技计划项目（MS12017013-4）；南通市卫计委青年项目（WKZL2018020）

Significance of Stromal Cell-Derived Factor 1, Chemokine Receptor 4 and Lymph Angiogenesis in Endometriosis

ZHOU Ya-juan，KAI Hai-li，QIAO Hai-feng，ZHANG Lian-suo，WU Liu-cheng，LIU Ying-lei，LIU Man-hua   

  1. Department of Obstetrics and Gynecology，Second Affiliated Hospital of Nantong University，Nantong 226001，Jiangsu Province，China（ZHOU Ya-juan，KAI Hai-li，QIAO Hai-feng，ZHANG Lian-suo，LIU Ying-lei，LIU Man-hua）；Experimental Animal Center of Nantong University，Nantong 226001，Jiangsu Province，China（WU Liu-cheng）
  • Received:2019-11-12 Revised:2020-02-28 Published:2020-04-15 Online:2020-04-15
  • Contact: LIU Ying-lei，E-mail：summerlyl622@163.com；LIU Man-hua，E-mail：liumanhua622@163.com E-mail:liumanhua2010@hotmail.com
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摘要： 目的：探讨基质细胞衍生因子1（SDF-1）、趋化因子受体4（CXCR4）及D2-40标记的微淋巴管密度（MLVD）在子宫内膜异位症（EMs）中的表达及意义。方法：选取2017年11月—2018年5月就诊于南通大学第二附属医院的卵巢型EMs患者20例，取其在位内膜和异位内膜。另选取同期因子宫肌瘤行子宫切除或子宫肌瘤挖除术患者20例，取其正常内膜。比较不同内膜组织中SDF-1、CXCR4 mRNA和蛋白的表达水平及MLVD，并通过细胞划痕实验验证SDF-1对异位子宫内膜细胞迁移能力的影响。结果：①异位内膜中SDF-1、CXCR4 mRNA和蛋白表达水平及MLVD均高于在位内膜、正常内膜，且在位内膜中的表达水平高于正常内膜，差异均有统计学意义（P＜0.05）。②SDF-1处理组细胞迁移率高于DMEM-F12处理组细胞迁移率，差异有统计学意义（P＜0.05）。结论：SDF-1、CXCR4可能参与EMs的淋巴管新生，促进子宫内膜细胞的淋巴迁移，在疾病的发生、发展中发挥一定作用。

关键词: 子宫内膜异位症；, 受体, CXCR4；, 淋巴管；, 淋巴转移；, 基质细胞衍生因子1

Abstract: Objective: To explore the expression and significance of stromal cell-derived factor 1 (SDF-1), chemokine receptor 4 (CXCR4) and D2-40 labeled microlymphatic vessel density (MLVD) in endometriosis (EMs). Methods: 20 patients with ovarian EMs who were treated in the Second Affiliated Hospital of Nantong University from November 2017 to May 2018 were selected, and eutopic and ectopic endometrium were taken. 20 patients who underwent hysterectomy or hysteromyomectomy removal during the same period were selected, and their normal endometrium was taken. The expression levels of SDF-1, CXCR4 mRNA and protein and MLVD in different endometrial tissues were compared. The effect of SDF-1 on the migration capacity of ectopic endometriotic cells was verified by cell scratching experiments. Results: ①The expression levels of SDF-1, CXCR4 mRNA and protein and MLVD in ectopic endometrium were higher than those in eutopic and normal endometrium, and those of eutopic endometrium was higher than those of normal endometrium, the differences were statistically significant (all P＜0.05). ②The cell migration rate of the SDF-1 treatment group was higher than that of the DMEM-F12 treatment group, and the difference was statistically significant (P＜0.05). Conclusions: SDF-1 and CXCR4 may be involved in lymphangiogenesis of EMs and promote lymphatic migration of endometrium, which plays an important role in the occurrence and development of diseases.

Key words: Endometriosis, Receptors, CXCR4;, Lymphatic vessels, Lymphatic metastasis;, Stromal cell-derived factor 1

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