国际妇产科学杂志

国际妇产科学杂志 ›› 2017, Vol. 44 ›› Issue (4): 443-447.

• 论著 • 上一篇    下一篇

寿胎丸对复发性流产小鼠蜕膜组织中螺旋动脉改变及怀孕结局的影响

许春月，郭洁，宋殿荣，赵琳，王雅楠，董胜雯   

  1. 300193  天津中医药大学（许春月）；天津中医药大学第二附属医院妇产科（郭洁，宋殿荣，赵琳，王雅楠，董胜雯）
  • 收稿日期:2017-04-21 修回日期:2017-07-14 出版日期:2017-08-15 发布日期:2017-08-15
  • 通讯作者: 郭洁，E-mail：guojie76@126.com E-mail:313124799@qq.com
  • 基金资助:
    寿胎丸调控人滋养层细胞增殖和迁移的作用机制研究

Study on Spiral Artery Transformation and Pregnancy Outcome of Recurrent Spontaneous Abortion Mice Treated with Shoutaiwan

XU Chun-yue，GUO Jie，SONG Dian-rong，ZHAO Lin，WANG Ya-nan，DONG Sheng-wen   

  1. Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China（XU Chun-yue）；Department of Obstetrics and Gynecology，Second Affiliated Hospital of Tianjin University of TCM，Tianjin 300143，China（GUO Jie，SONG Dian-rong，ZHAO Lin，WANG Ya-nan，DONG Sheng-wen）
  • Received:2017-04-21 Revised:2017-07-14 Published:2017-08-15 Online:2017-08-15
  • Contact: GUO Jie，E-mail：guojie76@126.com E-mail:313124799@qq.com
  • Supported by:
    天津市应用基础与前沿技术研究计划（14JCQNJC10400）

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摘要： 目的：通过观察寿胎丸对复发性流产（RSA）小鼠蜕膜组织子宫螺旋动脉改变及怀孕结局的影响，探讨其保胎的作用机制。方法：以CBA/J雌鼠×BALB/c雄鼠建立正常怀孕小鼠模型（12只），CBA/J雌鼠×DBA/2雄鼠建立RSA小鼠模型（43只），RSA小鼠按怀孕先后顺序随机分为RSA模型对照组（11只）、寿胎丸低剂量组（11只）、寿胎丸中剂量组（11只）及寿胎丸高剂量组（10只），于孕第4～9天灌胃给药，孕14 d处死孕鼠，计数吸收胚胎数，计算各组胚胎吸收率，光镜下观察各组蜕膜组织中螺旋动脉生理性改变的发生率，并测量螺旋动脉的管腔直径及管壁厚度，透射电镜下观察蜕膜组织中螺旋动脉超微结构。结果：RSA模型对照组胚胎吸收率高于正常怀孕模型组（29.49% vs. 4.55%，χ2=18.887，P=0.000）。寿胎丸低、中、高剂量组的胚胎吸收率比RSA模型对照组均降低（均P＜0.05）。RSA模型对照组蜕膜组织中子宫螺旋动脉生理性转变率低于正常怀孕模型组（11.90% vs. 44.68%， χ2=11.522，P=0.001）。RSA模型对照组蜕膜组织中子宫螺旋动脉平均管腔直径及管壁厚度均低于正常怀孕模型组（P＜0.05）。寿胎丸高剂量组子宫螺旋动脉生理性转变发生率增高，管腔扩张，管壁厚度变薄（均P＜0.05）。结论：寿胎丸可能通过促进RAS小鼠蜕膜组织中子宫螺旋动脉生理性重铸发挥保胎作用。
   

关键词:  寿胎丸, 流产, 习惯性, 复发性流产, 螺旋动脉

Abstract: Objectives: To observe the spiral artery transformation and pregnancy outcome of recurrent spontaneous abortion mice treated with Shoutaiwan, explore the mechanism of Shoutaiwan in mice with recurrent spontaneous abortion. Methods: The abortion-prone CBA/J×DBA/2 matings were established as the model of recurrent spontaneous abortion（43 mice） and the nonabortion-prone CBA/J×BALB/c matings were established as the model of normal pregnancy（12 mice）. The pregnant mice of CBA/J×DBA/2 were randomly divided into 4 groups. From the 4th to 9th day of pregnant mice of every group were oral administration in different doses. All pregnant mice were killed on the 14th day of pregnancy. Then counted the embryo absorption to calculate the absorption rate. The incidence of spiral arterial physiological changes in decidual tissue was observed under light microscope. And the diameter of lumen and wall thickness of spiral artery were measured. The ultrastructure of spiral artery in decidual tissue was observed under transmission electron microscope. Results: The embryo absorption rate of the recurrent spontaneous abortion model are higher than that of the normal pregnancy group (29.49% vs. 4.55%, χ2=18.887，P=0.000). The embryo absorption rate of low-dose, middle-dose and high-dose Shoutaiwan groups were significantly decreased than recurrent spontaneous abortion model group (P＜0.05). The spiral artery transformation rate of the recurrent spontaneous abortion model are lower than that of the normal pregnancy group (11.90% vs. 44.68%， χ2=11.522，P=0.001). Shoutaiwan could expand lumen diameter of the spiral artery and attenuate the thickness of spiral arteries wall. Conclusions: Shoutaiwan could promote the spiral artery transformation of recurrent spontaneous abortion mice.

Key words: Shoutai pill, Abortion, habitual, Recurrent spontaneous abortion, Spiral artery