国际妇产科学杂志

国际妇产科学杂志 ›› 2019, Vol. 46 ›› Issue (4): 382-386.

• 论著 • 上一篇    下一篇

机械生长因子对压力性尿失禁患者肛提肌修复作用的研究

董润楠，宋志英，王文渊   

  1. 030001 太原，山西医科大学研究生学院（董润楠）；山西省妇幼保健院妇产科（宋志英）；山西省肿瘤医院肛门结直肠科（王文渊）
  • 收稿日期:2019-01-11 修回日期:2019-04-24 出版日期:2019-08-15 发布日期:2019-08-15
  • 通讯作者: 宋志英，E-mail：1821682286@qq.com E-mail:1821682286@qq.com
  • 基金资助:
    山西省自然科学基金（2014011042-5）

Effect of Mechano Growth Factor on Levator Ani Muscle Repair in Patients with Stress Urinary Incontinence

DONG Run-nan，SONG Zhi-ying，WANG Wen-yuan   

  1. Shanxi Medical University Graduate School，Taiyuan 030001，China（DONG Run-nan）；Department of Obstetrics and Gynecology，Shanxi Maternal and Child Health Hospital，Taiyuan 030001，China（SONG Zhi-ying）；Department of Anal and Colorectal Surgery，Shanxi Cancer Hospital，Taiyuan 030001，China（WANG Wen-yuan）
  • Received:2019-01-11 Revised:2019-04-24 Published:2019-08-15 Online:2019-08-15
  • Contact: SONG Zhi-ying，E-mail：1821682286@qq.com E-mail:1821682286@qq.com

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摘要： 目的：通过检测女性压力性尿失禁（SUI）患者及直肠癌根治术患者肛提肌成肌细胞的存活率及层黏连蛋白（Laminin）、肌营养不良蛋白（Dystrophin）的表达情况，初步探索机械生长因子（MGF）对SUI患者的治疗作用。方法：原代分离女性SUI患者及直肠癌根治术患者的肛提肌成肌细胞，采用肌动蛋白免疫荧光方法对肛提肌成肌细胞进行鉴定，四甲基偶氮唑盐（MTT）法检测不同浓度MGF（0 ng/mL、50 ng/mL、100 ng/mL、150 ng/mL）在不同作用时间（24 h、48 h、72 h）下肛提肌成肌细胞的存活率，于成肌细胞孵育72 h后，采用反转录聚合酶链反应（RT-PCR）法测定上述两种蛋白的含量。结果：不同浓度MGF促进肛提肌成肌细胞增殖的效果不同（F浓度=65.055，P浓度=0.000）；不同MGF作用时间促进肛提肌成肌细胞增殖的效果不同（F时间=24.586，P时间=0.000）；MGF浓度和MGF作用时间两因素间存在交互作用（F时间×浓度=3.222，P时间×浓度=0.008），当MGF浓度为150 ng/mL和MGF作用时间为72 h时，肛提肌成肌细胞存活率最高。与对照组（直肠癌根治术患者的肛提肌成肌细胞）比较，SUI组（0 ng/mL）肛提肌成肌细胞Laminin、Dystrophin mRNA的表达量降低（P＜0.05）；与SUI组（0 ng/mL）比较，MGF组（150 ng/mL）肛提肌成肌细胞Laminin、Dystrophin mRNA的表达量增加（P＜0.05）。结论：MGF浓度和MGF作用时间两因素可交互作用，促进体外培养的SUI患者的肛提肌成肌细胞增殖。SUI患者肛提肌成肌细胞Laminin、Dystrophin的表达降低，MGF可促进SUI患者肛提肌成肌细胞Laminin、Dystrophin的表达。

关键词: 尿失禁, 压力性, 成肌细胞, 骨骼肌, 层黏连蛋白, 肌营养不良蛋白, 机械生长因子

Abstract: Objective: To explore the therapeutic effect of mechano growth factor (MGF) on patients with stress urinary incontinence (SUI) through detecting the survival rate of levator ani myoblasts and the expression of Laminin and Dystrophin in female patients with SUI and patients undergoing radical resection of rectal cancer. Methods：Levator ani myoblasts were isolated from female patients with SUI and patients undergoing radical resection of rectal cancer, and identified by actin immunofluorescence assay. The survival rate of levator ani myoblasts was determined by MTT assay using different concentrations of MGF (0 ng/mL, 50 ng/mL, 100 ng/mL, 150 ng/mL) at different time (24 h, 48 h, 72 h). After 72 hours incubation of myoblasts, the contents of these two proteins were determined by reverse transcription polymerase chain reaction (RT-PCR). Results：The effects of different concentrations of MGF on the proliferation of levator ani myoblasts were different (F浓度=65.055, P浓度=0.000). The effects of different action time of MGF on the proliferation of levator ani myoblasts were different (F时间=24.586, P时间=0.000). There was interaction between MGF concentration and MGF action time(F时间×浓度=3.222, P时间×浓度=0.008). When MGF concentration was 150 ng/mL and MGF action time was 72 h, the survival rate of levator ani myoblasts was the highest. Compared with the control group (levator ani myoblasts of patient undergoing radical resection of rectal cancer), the expression of Laminin and Dystrophin in levator ani myoblasts in SUI group (0 ng/mL) was lower (P＜0.05). Compared with the SUI group (0 ng/mL), the expression of Laminin and Dystrophin in levator ani myoblasts in MGF group (150 ng/mL) was higher (P＜0.05). Conclusions：The concentration of MGF and the action time of MGF can interact with each other to promote the proliferation of levator ani myoblasts in SUI patients cultured in vitro. The expression of Laminin and Dystrophin in levator ani myoblasts decreased in patients with SUI. MGF can promote the expression of Laminin and Dystrophin in levator ani myoblasts of SUI patients.

Key words: Urinary incontinence, stress, Myoblasts, skeletal, Laminin, Dystrophin, Mechano growth factor