国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (6): 692-694.

• 论著 • 上一篇    下一篇

OATP4A1、OATP2B1在妊娠期肝内胆汁淤积症胎盘表达的意义

王会敏   

  1. 442000 湖北省十堰市，湖北医药学院附属太和医院产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 王会敏

Expression and Significance of Organic Anion Transporting Polypeptides 4A1（OATP4A1），OATP2B1 in Placenta of Intrahepatic Cholestasis of Pregnancy

WANG Hui-min   

  1. Department of Obstetrics，Taihe Hospital Affiliated to Hubei University of Medical，Shiyan 442000，Hubei Province，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-12-15 Online:2014-12-15
  • Contact: WANG Hui-min

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摘要： 目的：探讨有机阴离子转运多肽4A1 （OATP4A1）、OATP2B1在妊娠期肝内胆汁淤积症（ICP）胎盘中表达的意义。方法：选择2011年8月—2012年8月在湖北医药学院附属太和医院产科行剖宫产的20例ICP患者为ICP组，选取同期因社会因素行剖宫产的正常晚期妊娠妇女20例为对照组。采用实时逆转录聚合酶链反应（real-time RT-PCR）检测2组妊娠妇女胎盘组织中OATP4A1、OATP2B1的mRNA表达水平，数据结果以荧光达到循环数（threshold cycle CT值）进行统计，数据分析采用2-△CT法，其中△CT=目标基因的CT值－内参的CT值。结果：①与对照组比较，ICP组的OATP4A1 mRNA表达水平上调（P＜0.05），OATP2B1的mRNA表达水平下调（P＜0.01）。②ICP组母血、脐血甘胆酸水平较对照组均明显升高（P＜0.01）。结论：ICP胎盘中OATP4A1的mRNA表达水平上调，可能与体内胆盐淤积，尤其是牛磺酸胆盐淤积有关，推测OATP4A1的表达上调在ICP发生中具有一定作用；OATP2B1的表达水平下调，可能与ICP体内雌激素代谢异常导致母体持续高水平雌激素刺激以及敏感性改变有关。

关键词: 胆汁淤积, 肝内, 剖宫产术, 聚合酶链反应, RNA, 信使, 蛋白质类, 雌激素类, 甘胆酸

Abstract: Objective：To investigate the expression and significance of organic anion transporting polypeptides 4A1（OATP4A1），OATP2B1 in placenta of intrahepatic cholestasis of pregnancy（ICP）. Methods：Twenty pregnant women with ICP were recruited and twenty normal cesarean section patients as control groups. The expression of mRNA was analyzed by real-time PCR. Data were analyzed by the comparative threshold cycle（CT） method and the 2-△CT method was used（△CT=CT gene of interest -CT internal control）. Results：The expression of OATP4A1 mRNA was higher（P＜0.05） and OATP2B1 was significantly lower（P＜0.01） in ICP placenta than in normal placenta. Significantly elevated cholylglycine（CG）′s level of fetal and maternal serum was associated with ICP. Conclusions：The data presented here suggests that up-regulation of transporter OATP4A1 may involve in the pathophysiology of ICP，which cause the disorder of bile acids. The expression of OATP2B1 in placenta was down-regulation in ICP. It may be involved in the disorder of estrogen metabolism in ICP，causing the mother continued high levels of estrogen stimulation and changed sensitivity.

Key words: Cholestasis, intrahepatic, Cesarean section, Polymerase chain reaction, RNA, messenger, Estrogens, Glycocholic acid