国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (1): 49-53.

• 论著 • 上一篇    下一篇

子宫内膜异位症中子宫内膜间质细胞血管生成能力的研究

苏晓华，宋殿荣，张英，张崴，郭洁，王雅楠，赵琳   

  1. 300193 天津中医药大学（苏晓华）；天津中医药大学第二附属医院（宋殿荣，张崴，郭洁，王雅楠，赵琳）；天津医科大学（张英）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-02-15 发布日期:2015-02-15
  • 通讯作者: 宋殿荣

Study of Angiogenesis Ability of Endometrial Stromal Cells in Endometriosis

SU Xiao-hua，SONG Dian-rong，ZHANG Ying，ZHANG Wei，GUO Jie，WANG Ya-nan，ZHAO Lin   

  1. Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China（SU Xiao-hua）；The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine，Tianjin 300150，China（SONG Dian-rong，ZHANG Wei，GUO Jie，WANG Ya-nan，ZHAO Lin）；Tianjin Medical University，Tianjin 300070，China（ZHANG Ying）
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-02-15 Online:2015-02-15
  • Contact: SONG Dian-rong

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摘要： 目的：研究子宫内膜异位症（EMs）患者子宫内膜间质细胞环氧化酶2（COX-2）、前列腺素E2（PGE2）、血管内皮生长因子（VEGF）的蛋白分泌及mRNA表达。方法：收集EMs患者的在位及异位病灶子宫内膜组织和子宫肌瘤患者在位内膜组织，原代消化培养子宫内膜细胞，取纯化的子宫内膜间质细胞进行研究，酶联免疫吸附测定法（ELISA）检测细胞COX-2、PGE2、VEGF蛋白的分泌，逆转录聚合酶链反应（RT-PCR）法检测COX-2、PGE2和VEGF的mRNA的表达。结果：EMs和子宫肌瘤患者分泌期在位子宫内膜间质细胞COX-2、PGE2、VEGF蛋白分泌及mRNA表达与增殖期比较差异均无统计学意义（P＞0.05）；EMs患者在位及异位的子宫内膜间质细胞COX-2、PGE2、VEGF蛋白分泌及mRNA表达比子宫肌瘤患者在位细胞均升高，差异有统计学意义（P＜0.05或P＜0.01）；EMs患者异位的子宫内膜间质细胞COX-2、PGE2、VEGF蛋白分泌及mRNA表达比其在位细胞均升高，差异有统计学意义（P＜0.05或P＜0.01）。结论：EMs患者在位及异位子宫内膜间质细胞COX-2、PGE2、VEGF的蛋白分泌及mRNA表达均升高，且异位病灶间质细胞升高更明显，提示在EMs发病中异位病灶子宫内膜间质细胞血管生成能力更强。

关键词: 子宫内膜异位症, 间质细胞, 环氧化酶2, 前列腺素E2, 血管内皮生长因子

Abstract: Objective： To study of the secretion and expression of COX-2，PGE2 and VEGF in endometrial stromal cells of patients with EMs. Methods：Ectopic endometrial tissues of EMs and eutopic endometrial tissues of patients with EMs or uterine fibroids were collected，then cultured endometrial cells by the methods of original digestion，the purified endometrial stromal cells were used for research，the secretion of COX-2，PGE2 and VEGF proteins were tested by ELISA，and the expression of COX-2，PGE2 and VEGF mRNA were detected by RT-PCR. Results：There were no statistically significant differences of the expression of COX-2，PGE2 and VEGF proteins and mRNA between the stage of secretion and proliferation in eutopic endometrial stromal cells in patients with EMs or uterine fibroid（P＞0.05）. The expression of COX-2，PGE2 and VEGF proteins and mRNA were significantly increased in eutopic and ectopic endometrial stromal cells of EMs compared with patients with uterine fibroid，the difference was statistically significant（P＜0.05 or P＜0.01）. The expression of COX-2，PGE2 and VEGF proteins and mRNA in ectopic endometrial stromal cells were obviously higher than that in eutopic endometrial stromal cells of EMs，the difference was statistically significant（P＜0.05 or P＜0.01）. Conclusions：The protein secretion and mRNA expression of COX-2，PGE2 and VEGF in eutopic and ectopic endometrial stromal cells were all increased and it showed that the ectopic cells increased more obviously. It indicated that the angiogenesis ability of ectopic endometrial stromal cells was stronger in the pathogenesis of EMs.

Key words: Endometriosis, Stromal cells, Cyclooxygenase 2, Prostaglandin E2, Vascular endothelial browth factors