国际妇产科学杂志

国际妇产科学杂志 ›› 2011, Vol. 38 ›› Issue (6): 585-587.

• 论著 • 上一篇    下一篇

河南地区妇女宫颈病变HPV型别分布研究

王晓静 , 刘玉玲, 汤福想, 王冬亮   

  1. 450014 郑州大学第二附属医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 刘玉玲

Distribution of Human Papillomavirus Types in Henan Women

WANG Xiao-jing，LIU Yu-ling，TANG Fu-xiang. WANG Dong-liang.   

  1. Department of Obstetrics and Gynecology，the Second Affiliated Hospital of Zhengzhou University，Zhengzhou 450014，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2011-12-15 Online:2011-12-15
  • Contact: LIU Yu-ling

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摘要： 目的：统计各型人乳头瘤病毒（HPV）在子宫颈癌和宫颈上皮内瘤变（CIN）Ⅱ以上病例中的分布数据，为HPV疫苗引入中国以及在中国开展HPV疫苗效果评价的四期临床试验提供基线资料。方法：采用“三明治”技术进行病理切片，并运用SPF10-聚合酶链反应（PCR）技术进行DNA扩增，再利用DNA酶免疫测定法（DEIA）和包括25种型别特异性探针的反向杂交线型探针检测技术（LiPA）对HPV阳性标本进行HPV分型检测。结果：91例宫颈组织样品（包括13例CINⅡ，36例CINⅢ，40例鳞状细胞癌，2例腺鳞癌），通过DEIA检测时，HPV均为阳性，阳性率100%；通过LiPA检测时，HPV阳性89例，阳性率97.8%，以HPV-16为主，其次是HPV-58，HPV-18和HPV-31。其中单一型感染72例，双重感染11例，多重感染6例。结论：河南地区宫颈病变患者组织中HPV感染主要为单一型感染，以HPV-16和HPV-58为主，为目前已研制成功的预防性疫苗在中国开展人群疫苗干预提供了理论基础，同时，HPV-58在研究人群中的重要性也对新型疫苗的开发提出挑战。

关键词: 人乳头瘤病毒16, 乳头状瘤病毒疫苗, 宫颈肿瘤, 聚合酶链反应, 宫颈上皮内瘤样病变

Abstract: Objective：Statistics in the distribution of data in all types of HPV and CINⅡ+ cases of cervical cancer in the distribution of data for HPV vaccine introduction in China and to provide baseline clinical trials for evaluation of the four HPV vaccine. Methods：SPF10-PCR，DEIA and LiPA technology were used to detect the type of HPV from paraffin-embedded cervical specimens. Results：It showed HPV positive in all the 91 paraffin-embedded cervical specimens by DEIA．And there are 89 postive cases by LiPA，among which 72 cases are single HPV type，17 cases are mixed HPV types. The top three prevalence of HPV types are follow：16，58，18. Conclusions：In Henan the most common HPV subtype is HPV 16 （80.9%），the second is HPV 58（10.1%）， the current preventive vaccine has been developed to carry out the crowd in our vaccine provides a theoretical basis for intervention，while 58 was the importance of the study population for novel vaccine development challenges.

Key words: Human papillomavirus 16, Papillomavirus vaccines, Uterine cervical neoplasms, Polymerase chain reaction, Cervical intraepithelial neoplasia