国际妇产科学杂志

国际妇产科学杂志 ›› 2011, Vol. 38 ›› Issue (6): 517-519.

• 论著 • 上一篇    下一篇

兔盆腔炎性疾病后遗症模型的制备

李俊良,刘 恒 ， 王建六，王世军， 魏丽惠   

  1. 100044 北京大学人民医院妇产科(李俊良现在清华大学附属第二医院工作)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-12-15 发布日期:2011-12-15
  • 通讯作者: 王建六

The Established Animal Model of Sequelae of Pelvic Inflammatory Disease

LI Jun-liang，LIU Heng，WANG Jian-liu，WANG Shi-jun，WEI Li-hui   

  1. Department of Obstetrics and Gynecology， Peking University People′s Hospital，Beijing 100044，China（LI Jun-liang now working at the Second Affiliated Hospital of Tsinghua University）
  • Received:1900-01-01 Revised:1900-01-01 Published:2011-12-15 Online:2011-12-15
  • Contact: WANG Jian-liu

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摘要： 目的：制备兔盆腔炎性疾病后遗症模型。方法：选用5～6个月龄未交配雌性兔5只，应用大肠埃希菌和金黄色葡萄球菌混合菌液采用逆行宫腔插管灌注细菌法建立兔盆腔炎性疾病后遗症模型。定期监测模型动物血常规、体温、体质量等指标，腹腔镜下观察兔盆腔炎症进展过程，取兔全子宫双输卵管行病理检查。结果：5只兔全部成功注入混合菌液，注射细菌3 d后血白细胞升高，14 d后下降并维持较低水平；注入细菌10～20 d左右兔体质量增加减缓甚至下降；腹腔镜观察，注入细菌14 d内，盆腔以充血、水肿及渗出为主要变化，14 d后以输卵管管壁增厚、输卵管增粗、挛缩为主要变化，经病理确认发生了盆腔炎症变化。结论：通过逆行子宫插管灌注细菌法可制备兔盆腔炎性疾病后遗症模型；该模型14 d左右为急性炎症期，14 d之后进入以增生、瘢痕修复为主要改变的盆腔炎性疾病后遗症期。

关键词: 盆腔炎性疾病, 兔, 疾病模型, 动物, 腹腔镜检查

Abstract: Objective：to established a rabbit model of sequelae of pelvic inflammatory disease. Methods：Female New Zealand white rabbits of 5-6 months old were studied with an intrauterine insemination catheter for bacterial inoculation for the model of PID. The blood routine，body temperature，body weight were measured. The uterine and oviduct were observed under laparoscopy. The pathological examination of the uterine and oviduct of the rabbits were done in this study. Results：The 5 rabbits were all be inoculated with bacterial successfully. The white blood cell count of the rabbits was increased at the third day and maintened after 14th day of bacterial inoculation. The body weight gain became slowly at 10-20 days，after that showed a steady upward trend. Under laparoscopic observation，edema and exudation were distinguished within 14 days. The tube wall became thickness，thickening，and contracture afterwards. Conclusions：The rabbit model of sequelae of rabbit pelvic inflammatory disease can be established with bacterial inoculation. The rabbit acute pelvic inflammation can be observed within 14 days. The modeling，proliferate and scar repair of rabbit pelvic inflammatory disease are the main changes after two weeks inoculated with bacterial.

Key words: Pelvic inflammatory disease, Rabbits, Disease models, animal, Laparoscopy