国际妇产科学杂志

国际妇产科学杂志 ›› 2012, Vol. 39 ›› Issue (5): 518-520.

• 论著 • 上一篇    下一篇

基因芯片筛选上调miRNA-205表达后HeLa细胞相关基因

韦丽娅, 姚婷婷, 陈庆瑜 , 沈青丽, 林仲秋   

  1. 510120 广州,中山大学孙逸仙纪念医院体检中心(韦丽娅、陈庆瑜),妇科肿瘤专科(姚婷婷、林仲秋);广州市妇婴医院妇产科(沈青丽)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-10-15 发布日期:2012-10-15
  • 通讯作者: 林仲秋

Analysis of Gene Expression Profile in HeLa Cells Upregulated Expression of MicroRNA-205 by cDNA Microarray

WEI Li-ya，YAO Ting-ting，CHEN Qing-yu，SHEN Qing-li，LIN Zhong-qiu   

  1. Medical Examination Center，Sun Yat-sen Memorial Hospital，Sun Yat-sen University，Guangzhou 510120，China （WEI Li-ya，CHEN Qing-yu）；Department of Gynecologic Oncology，Sun Yat-sen Memorial Hospital，Sun Yat-sen University，Guangzhou 510120，China（YAO Ting-ting，LIN Zhong-qiu）；Department of Gynaecology and Obstetrics，Guangzhou Women and Children′s Medical Center，Guangzhou 510180，China（SHEN Qing-li）
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-10-15 Online:2012-10-15
  • Contact: LIN Zhong-qiu

PDF (PC)

4945

被引次数

7

摘要： 目的：应用基因表达谱芯片技术筛查上调微小RNA-205（miR-205）表达后人宫颈腺癌细胞株HeLa细胞中差异表达基因，并探讨其功能及上调miR-205致癌机制。方法：应用含有23 232条人类全长基因的cDNA表达谱芯片，对上调miR-205表达的HeLa细胞及HeLa细胞原株的基因表达谱进行分析。结果：与HeLa细胞原株相比，上调miR-205表达的HeLa细胞中有差异表达的基因共172条，其中34条上调，138条下调。结论：基因芯片技术能够有效筛查出上调miR-205表达后HeLa细胞中差异表达基因，miR-205引发宫颈癌是多因素多基因共同作用的结果。

关键词: 宫颈肿瘤, HeLa细胞, 芯片分析技术, 微RNAs, 基因表达

Abstract: Objective：To identify a set of genes related to upregulated expression of miR-205 in HeLa cells and to investigate their function，and to discuss the pathogenesis of cervical cancer. Methods：The HeLa cells upregulated expression of miR-205 and normal HeLa cells were analyzed with 23 232 human genes of cDNA microarrays. Results：A total of 172 differential genes expressed in the HeLa cells upregulated expression of miR-205 were screened out，of which，34 were up regulated and 138 were down regulated. Conclusions：The cDNA microarray is a powerful method to identify the gene expression profile. Cervical cancer induced by upregulated expression of miR-205 is a result of coeffecting by polygene and multiple factors.

Key words: Uterine cervical neoplasms, Hela cells, Microchip analytical procedures, MicroRNAs, Gene expression