国际妇产科学杂志

国际妇产科学杂志 ›› 2013, Vol. 40 ›› Issue (1): 97-99.

• 论著 • 上一篇    下一篇

HHIP基因在子宫内膜腺癌中甲基化状态及表达


赵 欢, 冯玉珍, 庞义存   

  1. 050000 石家庄,河北医科大学第三医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-02-15 发布日期:2013-02-15
  • 通讯作者: 冯玉珍

Aberrant Methylation and the Expression of the Human Hedgehog Interacting Protein Gene in Endometrial Carcinoma

ZHAO Huan，FENG Yu-zhen，PANG Yi-cun   

  1. Department of Obstetrics and Gynecology，the Third Hospital of Hebei Medical University， Shijiazhuang 050000，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2013-02-15 Online:2013-02-15
  • Contact: FENG Yu-zhen

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摘要： 目的：检测HH相互作用蛋白（HHIP）基因在子宫内膜腺癌中的异常甲基化状态、其蛋白表达情况及两者的关系，探讨其在子宫内膜腺癌发生、发展过程中的作用机制。方法：收集子宫内膜腺癌新鲜组织标本30例和正常子宫内膜新鲜组织标本20 例，应用甲基化特异性聚合酶链反应（MS-PCR）方法检测HHIP基因的甲基化状态，并采用免疫组化法检测HHIP蛋白的表达情况，比较子宫内膜癌组织与正常子宫内膜组织HHIP基因甲基化状态、HHIP蛋白的表达情况，并分析HHIP基因甲基化与蛋白表达的相关性。结果：20例正常子宫内膜癌组织均为非甲基化状态，甲基化率为0（0/20），30例子宫内膜腺癌组织中，有12例发生了甲基化，甲基化率为40%（12/30），子宫内膜癌的甲基化率高于正常子宫内膜（P＜0.05）；HHIP蛋白在正常子宫内膜组织的表达均为阳性，表达率为100%（20/20），在子宫内膜腺癌组织中阳性表达率为40%（8/20），子宫内膜腺癌组织中存在HHIP蛋白的表达缺失情况，两者差异具有统计学意义（P＜0.05）。子宫内膜腺癌组织中甲基化阳性与甲基化阴性组织中HHIP蛋白表达率比较，差异无统计学意义（P=0.670）。结论：在子宫内膜腺癌中存有HHIP基因启动子区甲基化现象，并且存在HHIP蛋白表达的沉默；HHIP基因启动子区甲基化并非导致HHIP蛋白表达缺失的唯一因素，可能有其他机制导致子宫内膜腺癌中HHIP蛋白表达沉默。

关键词: 子宫内膜肿瘤, 腺癌, 甲基化, 免疫组织化学, 聚合酶链反应

Abstract: Objective：To investigate the aberrant methylation and the expression of Hedgehog Interacting Protein （HHIP） gene in endometrial carcinoma，and to explore the possible relationship between the methylation of the gene and the expression of the gene and their effect on the carcinogenesis and the proliferation of the endometrial carcinoma. Methods：The promoter methylation status of HHIP gene was tested by methylation-specific PCR in 20 normal endometrium tissus and 30 endometrial carcinoma tissus. The expression of HHIP was studied by histochemistry in 20 cases of normal endometrium and 30 cases of endometrial carcinoma.The correlation between the methylation status and endometrial carcinoma ，the correlation between the HHIP silencing and endometrial carcinoma as well as the correlation between the methylation status and the silencing of HHIP were tested by SPSS 13.0. Results：The frequencies of hepermethylation of HHIP in normal endometrium tissues were 0%（0/20），while the frequencies of heper-methylation of HHIP in endometrial carcinoma tissue were 40%（12/30）. The methylation rate in endometrial carcinoma tissue was higher than the methylation rate in normal endometrium tissue. All the normal endometrium tissues showed positive staining pattern of HHIP protein（20/20）. 40%（8/20） of endometrial carcinoma
tissues showed positive staining pattern of HHIP protein. In endometrial cancer， the HHIP protein silencing was easier to be found than in the normal endometrium tissue.The hepermethylation of HHIP gene was not related with the expression of HHIP protein（P＞0.05）. Conclusions：HHIP is related to the occurrence and the development of the endometrial carcinoma. The methylation rate in endometrial carcinoma tissue is higher than that in normal endometrium tissue. In endometrial cancer， the HHIP protein silencing is easier to be found than in the normal endometrium tissue. The methylation of the HHIP gene promoter may be related to the silencing of the expression of the protein to some extent，and it may be involved in the occurrence of the endometrial carcinoma. However， methylation may not be the only reason for the silencing of the expression of HHIP， and other mechanisms remain to be revealed.

Key words: Endometrial neoplasms, Adenocarcinoma, Methylation, Immunohistochemistry, Polymerase chain reaction