Journal of International Obstetrics and Gynecology

Journal of International Obstetrics and Gynecology ›› 2012, Vol. 39 ›› Issue (5): 518-520.

• 论著 • Previous Articles     Next Articles

Analysis of Gene Expression Profile in HeLa Cells Upregulated Expression of MicroRNA-205 by cDNA Microarray

WEI Li-ya，YAO Ting-ting，CHEN Qing-yu，SHEN Qing-li，LIN Zhong-qiu   

  1. Medical Examination Center，Sun Yat-sen Memorial Hospital，Sun Yat-sen University，Guangzhou 510120，China （WEI Li-ya，CHEN Qing-yu）；Department of Gynecologic Oncology，Sun Yat-sen Memorial Hospital，Sun Yat-sen University，Guangzhou 510120，China（YAO Ting-ting，LIN Zhong-qiu）；Department of Gynaecology and Obstetrics，Guangzhou Women and Children′s Medical Center，Guangzhou 510180，China（SHEN Qing-li）
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-10-15 Online:2012-10-15
  • Contact: LIN Zhong-qiu

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Abstract: Objective：To identify a set of genes related to upregulated expression of miR-205 in HeLa cells and to investigate their function，and to discuss the pathogenesis of cervical cancer. Methods：The HeLa cells upregulated expression of miR-205 and normal HeLa cells were analyzed with 23 232 human genes of cDNA microarrays. Results：A total of 172 differential genes expressed in the HeLa cells upregulated expression of miR-205 were screened out，of which，34 were up regulated and 138 were down regulated. Conclusions：The cDNA microarray is a powerful method to identify the gene expression profile. Cervical cancer induced by upregulated expression of miR-205 is a result of coeffecting by polygene and multiple factors.

Key words: Uterine cervical neoplasms, Hela cells, Microchip analytical procedures, MicroRNAs, Gene expression