Abstract: Objective：To explore the expressions of maspin and p53 in gestational trophoblastic disease, and their roles in the development of hydatidiform mole. Methods：The expressions of maspin and p53 were detected by immunohistochemistry （PV9000） in 22 cases of normal early pregnancies, 82 cases of hydatidiform moles （35 cases were followed up to occur malignant transformation） and 11 cases of gestational trophoblastic tumors. Results：Hydatidiform mole without malignant transformation group and normal early pregnancy group, hydatidiform mole with malignant group and hydatidiform mole without malignant transformation group, the positive expression rate of maspin was significantly lower, but the positive expression rate of p53 was significantly higher, the difference was statistically significant （P＜0.05）. In moles with malignant high risk factors such as β-hCG＞105 U/L, uterus volume ＞ gestation weeks, ovary lutein cyst ＞6 cm, the expression of maspin was lower than those groups such as β-hCG≤105 U/L，uterus volume ≤ gestation weeks, ovary lutein cyst ≤6 cm （P＜0.05）, while the expression of p53 was higher in moles with malignant high risk factors such as β-hCG＞105 U/L，ovary lutein cyst ＞6 cm compared with those without risk factors groups （P＜0.05）. Maspin expression was correlated with p53 （P＜0.05）. In 35 cases of malignant moles the expression of maspin in FIGO prognostic score ≥7 was lower than that in FIGO prognostic score ＜7（P＜0.05）. The expression of p53 in anatomy staging Ⅲ group was higher than that in anatomy staging Ⅰ-Ⅱ group （P＜0.05）. Conclusions：Decreased expression of maspin and high expression of p53 may be early events in the malignant transformation of hydatidiform mole. It is suggested that the joint detection of both proteins provide certain reference value in predicting hydatidiform mole malignant transformation and prognostic evaluation of gestational trophoblastic disease.

Key words: maspin, p53, Hydatidiform mole, Choriocarcinoma, Immunohistochemistry