Journal of International Obstetrics and Gynecology

Journal of International Obstetrics and Gynecology ›› 2009, Vol. 36 ›› Issue (5): 408-410.

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The Study of the Nuclear Red Blood Cells′ Rich by Interfering Membrane Ionic Channels Selectively

CHENG Ning;ZHANG Li-na;LIU Fang;XU Xiang-hong;Ya-na   

  1. Center of Reproductive Health and Birth Defects, Lanzhou University, Lanzhou 730000, China（CHENG Ning, ZHANG Li-na, LIU Fang, XU Xiang-hong）; Institute of Epidemiology and Statistics, Medical School, Lanzhou University, Lanzhou 730000, China（BAI Ya-na）; Gansu Province Key Laboratory of Preclinical Study for New Medicine, Lanzhou University, Lanzhou 730000, China（CHENG Ning）
  • Received:2009-03-12 Revised:1900-01-01 Published:2009-10-15 Online:2009-10-15
  • Contact: CHENG Ning

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Abstract: Objective: To investigate the effectiveness of the Nuclear Red Blood Cells ′ rich by effecting
membrane ionic channels-Anion Exchanger and Na+/K+/2Cl- Co-transporter in umbilical cord blood. Methods: Make the optimal
interfering condition from RBCs on the umbilical cord blood，the rate of cells′ enrichment by discontinuous density
centrifugation on different densities were determined by Flow Cytometry（FCM）. Results: The average purity of NRBCs
enrichment in the umbilical cord blood could take from 2.4% to 9.7% （u=3.19, P<0.05） by interfering, which means to increase
about 4 times of enrichment of NRBC. Conclusions: Both Verapamil and Furosemide could promote the rate of NRBC′enrichment
about 4 times.

Key words: Nuclear red blood cells, Ionic channels, Verapamil, Furosemide, Interfering, Flow cytometry