Abstract: Objective：To evaluate the signification of serum soluble fms-like tyrosine kinase－１（ｓＦｌｔ－１），placental growth factor（ＰlＧＦ），25-hydroxyvitamin D（25-OH-VD），D-dimer，von Willebrand factoｒ（ｖＷＦ），P-selectin for predicting risk of pre-eclampsia（PE） during the first trimester of pregnancy. Methods：In this nested cohort ，we recruited 1 081 consecutive women who presented for antenatal care between 10 and 13+6 weeks of gestation from Shenzhen Maternity and Child Healthcare Hospital from January 2013 to December 2013. Of the 1 081 pregnant women studied，50 patients were diagnosed with PE as case group. There are 352 normal pregnant women without complications as control group. Serum sFlt-1，PLGF，25-OH-VD，D-dimer，vWF and P-selectin were detected by ELISA. Using ROC curve to evaluate the value of diagnosis preeclampsia for the six index alone or combination. Results：During 10-13+6 weeks of gestation serum levels of sFlt-1，D-dimer，vWF，P-selectin of gravida with PE were significantly higher than the control group （P＜0.05）. The serum PlGF and 25-OH-VD levels in case group was significantly lower than that in the control group（P＜0.05）. Combining total multiple of median （MoM） of serum sFlt-1，PlGF，25-OH-VD，D-dimer，vWF，P-selectin using ROC curve analysis can get a sensitivity of 78% and a specificity of 100% for predicting the risk of PE. Conclusions：Combining screening of different biomarkers such as serum sFlt-1，PlGF,25-OH-VD，D-dimer，vWF and P-selectin could increase the predictive value of PE at the first trimester pregnancy.

Key words: Pre-eclampsia, Vascular endothelial growth factor receptor-1, Placental growth factor, Calcifediol, D-dimer, von Willebrand factor, Selectins