Journal of International Obstetrics and Gynecology

Journal of International Obstetrics and Gynecology ›› 2017, Vol. 44 ›› Issue (2): 163-166.

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The Effects of Inhibiting CXCR7 Gene Expression on Migration and Invasion of HeLa Cells and Expression of Cervical Cancer Tissues

WANG Jing-wen，WANG Si-nian，SUN Suo-zhu，ZHANG Xin，YU Nan，LI Feng-sheng   

  1. Graduate School，Liaoning University of Traditional Chinese Medicine，Shenyang 110032，China（WANG Jing-wen）；Laboratory of Nuclear and Radiation Damage（WANG Si-nian，YU Nan，LI Feng-sheng），Department of Pathology（SUN Suo-zhu），The General Hospital of the PLA Rocket Force，Beijing 100088，China；Cancer Hospital of China Medical University，Liaoning Cancer Hospital and Institute，Shenyang 110042，China（ZHANG Xin）
  • Received:2017-02-13 Revised:2017-03-22 Published:2017-04-15 Online:2017-05-18
  • Contact: ZHANG Xin，E-mail：zhangxiangmiao@hotmail.com；LI Feng-sheng，E-mail：lifs0624@163.com E-mail:wjw414@qq.com

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Abstract: 【Abstract】  Objective：To investigate the effect of chemokine receptor 7 (CXCR7) silencing on invasion and migration of HeLa cells and its expression in cervical carcinoma and normal cervical tissues. Methods：Respectively, after the HeLa cells were transfected with plasmid expressing CXCR7 shRNA (CXCR7-shRNA) or negative control shRNA (Ctrl-shRNA), detecting the changes in cell migration and invasion of them by Transwell method. Immunohistochemistry was used to detect the expression of CXCR7 in cervical cancer tissues and non cancerous cervical tissues. Results：CXCR7 protein was positive in 16 cases of cervical cancer tissrues. Transwell invasion and migration assay showed that the number of CXCR7-shRNA transfected group was significantly lower than that of Ctrl-shRNA transfection group and non transfection group. Conclusions：The expression of CXCR7 was significantly higher in cervical cancer tissues, and the expression of CXCR7 could inhibit the invasion and migration of cervical cancer cells. Therefore, CXCR7 may be an important target for the prevention and treatment of cervical cancer.
【Keywords】 

Key words: Receptors, chemokine, Hela cells, Neoplasm invasiveness, Cell movement