国际妇产科学杂志

国际妇产科学杂志 ›› 2014, Vol. 41 ›› Issue (2): 201-203.

• 论著 • 上一篇    下一篇

胆固醇对滋养细胞凋亡及尿酸产生的影响

李柳叶，黄亚绢   

  1. 215006 江苏省苏州市，苏州大学（李柳叶）；上海市第六人民医院妇产科（黄亚绢）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-04-15 发布日期:2014-04-15
  • 通讯作者: 黄亚绢

The Impact of Cholesterol on Apoptosis and the Production of Uric Acid of Trophoblasts Cells

LI Liu-ye，HUANG Ya-juan   

  1. Soochow University，Suzhou 215006，Jiangsu Province，China（LI Liu-ye）；Department of Obstetrics and Gynecology，the Sixth People Hospital of Shanghai，Shanghai 200233，China（HUANG Ya-juan）
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-04-15 Online:2014-04-15
  • Contact: HUANG Ya-juan

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摘要： 目的：观察不同浓度胆固醇对滋养细胞凋亡及尿酸产生的影响。方法：不同胆固醇浓度培养基培养滋养细胞（HPT-8系）24 h后，用Annexin V/PI双染色法检测不同胆固醇浓度培养基中滋养细胞的凋亡程度。同时用酶联免疫吸附（ELISA）法检测不同胆固醇浓度培养滋养细胞24 h后上清液中尿酸的浓度。结果：通过检测分析发现，培养基中胆固醇浓度（0，25，50，100 mg/L）越高，滋养细胞凋亡率越高，细胞培养上清液中的尿酸浓度也越高，且呈现出剂量依赖性。结论：培养基中胆固醇浓度越高，引起滋养细胞凋亡的能力越强，从而导致滋养细胞微粒的数量增加。同时高胆固醇浓度也会引起滋养细胞产生尿酸的水平增加，引起系统炎症反应及血管内皮系统功能的紊乱，从而在子痫前期的发病过程中发挥作用。

关键词: 先兆子痫, 胆固醇, 坏死, 细胞凋亡, 尿酸

Abstract: Objective：To study the influence of different concentrations of cholesterol on cell apoptosis and producing uric acid of trophoblast cell. Methods：Primary culture trophoblasts cells （HPT-8） in the medium with different concentrations of cholesterol for 24 hours，then Annexin V/PI method were used to detect different cells′ apoptosis. Meanwhile，we used ELISA to detect uric acid concentration in the supernatant after 24 hours. Results：Through the analysis we found that the higher cholesterol concentrations （0，25，50，100 mg/L） in medium，the higher cell apoptosis rate. The level of uric acid in the cell culture supernatant is higher，and presents a dose dependent. Conclusions：When medium cholesterol concentration become high，it cause more serious apoptosis of cells，which leads to increased number of microparticles. At the same time the high cholesterol concentration can also cause increased production of uric acid，then induce system inflammatory response and endothelal dysfunction. The two factors play important roles in the pathogenesis of preeclampsia.

Key words: Pre-eclampsia, Cholesterol, Necrosis, Apoptosis, Uric acid