国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (1): 112-115.

• 论著 • 上一篇    下一篇

Hes1对宫颈癌细胞转移及上皮间质转化的影响

李睿歆，姚婷婷，刘昀昀，谢庆生，谢晓飞，梁金晓，林仲秋   

  1. 510120 广州，中山大学孙逸仙纪念医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-02-15 发布日期:2015-02-15
  • 通讯作者: 林仲秋

Effects of Hes1 in Metastasis and Epithelial-Mesenchymal Transition in Cervical Cancer Cells

LI Rui-xin，YAO Ting-ting，LIU Yun-yun，XIE Qing-sheng，XIE Xiao-fei，LIANG Jin-xiao，LIN Zhong-qiu   

  1. Department of Obstetrics and Gynecology，Sun Yat-sen Memorial Hospital，Sun Yat-sen University，Guangzhou 510120，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-02-15 Online:2015-02-15
  • Contact: LIN Zhong-qiu

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摘要： 目的：研究Hes1在宫颈癌细胞迁移、侵袭及上皮间质转化中的作用。方法：实时荧光定量聚合酶链反应（PCR）、蛋白质印迹（Western blotting）检测宫颈癌4个细胞系Hes1表达。小干扰RNA（siRNA）干扰下调Hes1后，Transwell检测Hes1下调对宫颈癌细胞迁移、侵袭能力的影响。通过实时荧光定量PCR、蛋白质印迹法检测Hes1下调对上皮间质转化（EMT）主要相关分子的影响。结果：Hes1 mRNA在4种宫颈癌各细胞系中的表达差异有统计学意义（χ2=10.39，P=0.016）。下调Hes1后，迁移实验和侵袭实验均显示，穿膜细胞数SiHa NC组与SiHa 1组、HeLa NC组与HeLa 1组差异有统计学意义（均P＜0.05）。Hes1下调后，与空白对照组、阴性对照组比较，SiHa及HeLa的E-cadherin表达量均减少（均P＜0.05），SiHa细胞ZEB1、Slug表达量增加（均P＜0.05）；HeLa细胞vimentin、Slug表达量增加（均P＜0.05）。结论：宫颈癌SiHa、HeLa细胞中Hes1抑制迁移、侵袭；Hes1下调引起EMT；Hes1可能通过EMT参与宫颈癌细胞的迁移和侵袭。

关键词: 宫颈肿瘤, 细胞运动, 肿瘤侵润, 上皮间质转化, Hes1

Abstract: Objective：To investigate the role of Hes1 in migration, invasion and epithelial-mesenchymal transition in cervical cancer cells. Methods：Real time quantitative PCR（RT-qPCR） and Western blotting were used to detect the expression of Hes1 in four cervical cancer cell lines. Hes1 knockdown was performed by siRNA. The effects of down-regulation of Hes1 on migration and invasion in cervical cancer cells were evaluated by transwell assays. The main factors of epithelial-mesenchymal transition effected by Hes1 knock down were measured by RT-qPCR and Western blotting. Results：The expressions of Hes1 mRNA in four cervical cancer cell lines showed statistic differences （ χ2=10.39，P=0.016）. Knockdown of Hes1 led to statistic differences in the numbers of cell passing through the chamber, between SiHa NC group and SiHa 1 group, HeLa NC group and HeLa 1 group（P＜0.05, both） in both migration assays and invasion assays. After Hes1 RNA interference, compared to blank and negative control, the expression of E-cadherin was significantly reduced in SiHa and HeLa（both P＜0.05）, while ZEB1，Slug were induced in SiHa（both P＜0.05）, and vimentin, Slug were induced in HeLa（both P＜0.05）. Conclusions：Hes1 may have a role in suppressing migration and invasion in SiHa, HeLa cells, and subsequent down-regulation induces the epithelial-mesenchymal transition, thereby suggesting a possible role in migration and invasion of cervical cancer cells mediated by epithelial-mesenchymal transition.

Key words: Uterine cervical neoplasms, Cell movement, Neoplasm invasiveness, Epithelial-mesenchymal transition, Hes1