国际妇产科学杂志

国际妇产科学杂志 ›› 2015, Vol. 42 ›› Issue (2): 161-164.

• 论著 • 上一篇    下一篇

miR-199a在卵巢癌中表达下调及其对卵巢癌生物学功能的影响

张越，王落巧，林其德，张殊   

  1. 200127 上海交通大学医学院附属仁济医院妇产科，上海市妇科肿瘤重点实验室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-04-15 发布日期:2015-04-15
  • 通讯作者: 张殊

The Down-Regulation of miR-199a in Ovarian Cancer and Its Effect on Ovarian Cancer Biological Functions

ZHANG Yue，WANG Luo-qiao，LIN Qi-de，ZHANG Shu   

  1. Department of Obstetrics and Gynecology， Renji Hospital，School of Medicine，Shanghai Jiao Tong University， Shanghai Key Laboratory of Gynecologic Oncology, Shanghai 200127，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-04-15 Online:2015-04-15
  • Contact: ZHANG Shu

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摘要： 目的：检测miR-199a在上皮性卵巢癌组织中的表达水平以及在卵巢癌中参与的功能，初步探讨miR-199a在卵巢癌发生、发展中的作用和机制。方法：通过实时定量逆转录聚合酶链反应（qRT-PCR）检测上皮性卵巢癌20例、良性上皮性卵巢肿瘤组织15例以及正常卵巢上皮细胞IOSE和1组具有不同侵袭能力的卵巢癌细胞株中miR-199a的表达；构建miR-199a的逆转录病毒质粒pMSCVpuro-miR-199a并筛选稳定过表达miR-199a的SKOV3细胞株；分别用CCK8和Transwell小室法检测过表达miR-199a的SKOV3细胞在增殖、侵袭及迁移方面的改变。结果：miR-199a在卵巢癌组的表达低于良性卵巢肿瘤组（P=0.001），miR-199a在卵巢癌细胞中的表达低于正常卵巢上皮细胞IOSE（P＜0.05），且在SKOV3中表达最低（P＜0.01）；与转染空载质粒SKOV3细胞相比，稳定转染pMSCVpuro-miR-199a质粒的SKOV3细胞增殖、侵袭和迁移力均降低（P＜0.000 1）。结论：miR-199a在卵巢癌组织中表达降低，并在卵巢癌细胞增殖、侵袭及迁移过程中起抑癌基因的作用。

关键词: 卵巢肿瘤, 微RNAs, 细胞增殖, 细胞运动, 肿瘤侵润

Abstract: Objective： To study the expression and potential function of miR-199a in ovarian cancer occurrence and progression. Methods：Quantitative reverse transcriptase PCR（qRT-PCR） was used to detect the expression of miR-199a in 20 cases of epithelial ovarian cancers, 15 cases of benign epithelial ovarian tumors, ovary normal epithelial cell （IOSE） and ovarian cancer cells. Retrovirus expression vector pMSCVpuro-miR-199a was constructed and SKOV3 cells were transfected. CCK8 and transwell methods were used to detect the ability of proliferation and metastasis in SKOV3 cells after stably transfected with pMSCVpuro-miR-199a. Results：The mRNA expression of miR-199a in epithelial ovarian cancer group was significantly lower than that of in benign ovarian tumors （P=0.001）. Compared with IOSE cells，the miR-199a expression were down-regulated in ovarian cancer cells and expecially in SKOV3 cells（P＜0.01）. The abilities of proliferation，migration and invasion in SKOV3 cells transfected with pMSCVpuro-miR-199a were significantly lower than that in pMSCVpuro-NC SKOV3 cells（P＜0.000 1）. Conclusions：Our data suggest that the expression of miR-199a was down-regulated in ovarian cancer and miR-199a might play as an anti-oncogene in ovarian cancer proliferation and metastasis.

Key words: Ovarian neoplasms, MicroRNAs, Cell proliferation, Cell movement, Neoplasm invasiveness