国际妇产科学杂志

国际妇产科学杂志 ›› 2017, Vol. 44 ›› Issue (6): 690-694.

• 论著 • 上一篇    下一篇

应用飞行时间质谱分析卵巢癌相关肿瘤标记物

刘贵芬，宋一一，孙蓬明，阮冠宇   

  1. 350001  福州，福建省妇幼保健院，福建医科大学附属医院
  • 收稿日期:2017-09-04 修回日期:2017-10-13 出版日期:2017-12-15 发布日期:2017-12-15
  • 通讯作者: 宋一一，E-mail：fjyiyisong@sina.com E-mail:1825699239@qq.com
  • 基金资助:
    2014年福建省自然科学基金项目（2014J01278）；2015年福建省卫生系统中青年骨干人才培养项目（2014-ZQN-ZD-5）

Application of Time-of-flight Mass Spectrometry for Serum Biomarkers in Ovarian Cancer

LIU Gui-fen，SONG Yi-yi，SUN Peng-ming，RUAN Guan-yu   

  1. Fujian Provincial Maternity and Children′s Hospital，Affiliated Hospital of Fujian Medical University，Fuzhou 350001，China
  • Received:2017-09-04 Revised:2017-10-13 Published:2017-12-15 Online:2017-12-15
  • Contact: SONG Yi-yi，E-mail：fjyiyisong@sina.com E-mail:1825699239@qq.com

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摘要： 目的：利用纳米磁珠与基质辅助激光解吸离子飞行时间质谱（MALDI-TOF-MS）技术检测卵巢癌患者血清蛋白，建立血清诊断模型及筛选潜在的肿瘤标志物。方法：正常对照组8例，良性肿瘤22例，交界性肿瘤16例，卵巢恶性肿瘤16例，用弱阳离子交换型（WCX）纳米磁珠检测各血清标本获得血清蛋白指纹图谱，用Biomarker Wizard软件分析所获得的蛋白图谱找出差异蛋白。同时与CA125的敏感度和特异度做比较。结果：m/z为4 508.71可以区别正常人群与交界性卵巢肿物，其敏感度为100%，特异度为62.5%，同时也能将卵巢交界性肿瘤与良性肿瘤区分出来，其敏感度和特异度分别为83.3%、72.8%。另外m/z为2 665.32能将卵巢交界性肿瘤和恶性肿瘤区别开来，其敏感度和特异度分别为100%和50.0%，进一步与常见的肿瘤标志物CA125（敏感度为76%）相比较，其鉴别交界性卵巢肿物方面敏感度更高（与正常对照组比较敏感度为100%，与良性肿瘤组比较为83.3%）。结论：MALDI-TOF-MS技术不仅能够建立诊断模型，还能够筛选出对鉴别卵巢肿物有意义的血清标志差异蛋白。
 

关键词:  卵巢肿瘤, 气相色谱-质谱法, 肿瘤标记, 生物学, 基质辅助激光解吸离子飞行时间质谱

Abstract: Objective：To analyze the serum biomarkers of the ovarian cancer patients, to establish serum diagnosis models and to find potential biomarkers with MALDI-TOF-MS combined with WCX magnetic bead. Methods：8 healthy controls, 22 benign ovarian tumor, 16 borderline tumor, and 16 ovarian cancer patients were enrolled to pre-extract the serum samples using the MB-WCX kit. Then, Biomarker Wizard software was used to detect protein peaks and potential difference between these groups. Last comparing with CA125 in sensitivity and specificity. Results：A diagnostic pattern for borderline tumor from healthy controls using m/z 4 508.71 was established with 100% sensitivity and 62.5% specificity, for borderline tumor from benign healthy controls was 83.3% sensitivity and 72.8% specificity, and m/z 2 665.32 for ovarian cancer from borderline tumor was established with 100% sensitivity and 50.0% specificity. Further compared to traditional CA125 (sensitivity 76%), the sensitivity of this methods is higher (compared with normal control group was 100%, compared with benign tumor group of 83.3%). Conclusions：MALDI-TOF-MS can be used not only to establish serum diagnosis models, but also to search for potential biomarkers in ovarian cancer patients′ serum samples.
【Keywords】 

Key words:  Ovarian neoplasms, Gas chromatography-mass spectrometry, Tumor markers, biological, Matrix-assisted laser desorption ionization time-of-flight mass spectrometry